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A note on sample preparation in the analysis of nitrate and nitrite in forage

Spörndly, Rolf and Grönqvist, Tomas and Knicky, Martin and Ericson, Börje (2016). A note on sample preparation in the analysis of nitrate and nitrite in forage. I/In: 7th Nordic Feed Science Conference, Uppsala.
[Conference Paper]

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Abstract

Nitrate content in forage is of interest as it pose a risk of poisoning for ruminants at levels above 1000 mg NO3-N per kg DM (Strickland et al, 1995; Undersander et al, 1999).
Analysing nitrate in forages by colorimetric methods have been questioned during decades due to interference of coloured substances in the forage (Wiseman & Jacobson, 1962; Wegner, 1972; Anderson & Case, 1999). Nevertheless, the colorimetric method using flow injection analysis (FIA) with a Cu-Cd reduction column has become a standard method in analysing nitrite and nitrate after reduction to nitrite (MacKown & Weik, 2004). The problem of diverging results in nitrate analysis has often appeared as a result of different nitrate extraction techniques, as opposed to differences due to detection methods after extraction (Anderson & Case, 1999). The sample preparation and analytical method in use at the departments of Soil and Environment, Crop Production Ecology and Animal Nutrition and Management at the Swedish University of Agricultural Science has been water extraction of dried samples followed by the nitrite and nitrate analysis according to the ISO method 13395;1996. As nitrate, and in particular nitrite, are unstable compounds, doubts have been raised concerning the influence of the sample preparation step. The present study was made with the aim to compare result of nitrite and nitrate analyses of dried or un-dried samples and extraction with boiling water or room tempered water.

Authors/Creators:Spörndly, Rolf and Grönqvist, Tomas and Knicky, Martin and Ericson, Börje
Title:A note on sample preparation in the analysis of nitrate and nitrite in forage
Series/Journal:Rapport / Sveriges lantbruksuniversitet, Institutionen för husdjurens utfodring och vård (0347-9838)
Year of publishing :2016
Number:293
Page range:17-20
Number of Pages:4
Place of Publication:Uppsala
Publisher:SLU, Sveriges lantbruksuniversitet
ISSN:0347-9838
Language:English
Publication Type:Conference Paper (Paper)
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:nitrate content, forage, analyses
URN:NBN:urn:nbn:se:slu:epsilon-e-4805
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4805
ID Code:15044
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:13 Mar 2018 09:10
Metadata Last Modified:26 Aug 2020 22:54

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