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The mitochondrial influence on nuclear gene expression in cytoplasmic male-sterile Brassica napus

Carlsson, Jenny (2007). The mitochondrial influence on nuclear gene expression in cytoplasmic male-sterile Brassica napus. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2007:68
ISBN 978-91-576-7367-1
[Doctoral thesis]

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Abstract

Phenotypic, genetic and molecular studies were made on Brassica napus (Arabidopsis thaliana) lines carrying mitochondria with DNA from both B. napus and A. thaliana. The lines were isogenic regarding the nuclear and chloroplast genomes, which consisted of B. napus DNA. Most lines contained large but variable portions of A. thaliana mitochondrial DNA detected by a dense set of A. thaliana specific markers. Several of the A. thaliana sequences gave rise to novel transcripts. The lines were divided into three classes; male-sterile, semi-sterile and fertile according to their stamen morphology. The flowers of the male-sterile lines were characterized by replacement of stamens with carpeloid organs, which resemble the apetala3 (ap3) and pistillata (pi) mutants found in A. thaliana. The AP3 and PI gene expression were lower in the male-sterile lines. The AP3 expression was down-regulated in the stamens shortly before these organs developed carpeloid characteristics. Repression of PI succeeded that of AP3 and might be a consequence of loss of AP3 activity. Low levels of AP3 and PI proteins were found in a male-sterile line. These results suggest that AP3 expression in stamens depends on proper mitochondrial function and correct nuclear–mitochondrial interactions. To study the nuclear gene expression profiles of flower buds in the lines, two different types of microarrays were used. Gene expression profiling revealed that a large number of genes differed in expression between the lines. These results show that the mitochondrial genome strongly influences nuclear gene expression and reveal the importance of retrograde signalling between the mitochondria and the nucleus. In addition, the mitochondria directly or indirectly influence several different aspects of plant development and metabolism, since not only flower morphology but also growth rate, flowering time and adenylate content were affected. An additional CMS-system, B. napus (Ogu-INRA) was also studied. The conclusion is that the two CMS-systems differ in expression at stage 8, likely due to timing. The nuclear gene expression of the B. napus (A. thaliana) CMS-line was altered already at stages 0-5 while the expression of the Ogu-INRA CMS-line was altered at stage 8. This was reflected in the phenotypes of the two different CMS-lines.

Authors/Creators:Carlsson, Jenny
Title:The mitochondrial influence on nuclear gene expression in cytoplasmic male-sterile Brassica napus
Year of publishing :2007
Volume:2007:68
Number of Pages:58
Papers/manuscripts:
NumberReferences
ALLI. Carlsson, J., Leino, M. & Glimelius, K. 2007. Mitochondrial genotypes with variable parts of Arabidopsis thaliana DNA affect development in Brassica napus lines. Theoretical and Applied Genetics DOI 10.1007/s00122-007-0593-2. II. Carlsson, J., Lagercrantz, U., Sundström, J., Teixeira, R., Wellmer, F., Meyerowitz, E.M. & Glimelius, K. 2007. Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers. The Plant Journal 49, 452-462. III. Carlsson, J., Bitton, F., Renou, J-P., Budar, F. & Glimelius, K. The impact of novel mitochondrial genomes on nuclear gene expression in two Brassica napus CMS-systems. (manuscript). IV. Sundström, J.F., Carlsson, J., & Glimelius, K. Mitochondrial regulation of the nuclear encoded transcription factors APETALA3 and PISTILLATA in a Brassica napus CMS-system. (manuscript).
Place of Publication:Uppsala
ISBN for printed version:978-91-576-7367-1
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:brassica napus, arabidopsis thaliana, mitochondria, cytoplasmic male sterility, homeotic genes, flowering, gene expression
Keywords:APETALA3, flower development, homeotic genes, male-sterility, microarray, mitochondrial gene expression, PISTILLATA, restoration of fertility, retrograde signalling, somatic hybrids
URN:NBN:urn:nbn:se:slu:epsilon-1751
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1751
ID Code:1518
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: Jenny Carlsson
Deposited On:24 Aug 2007 00:00
Metadata Last Modified:02 Dec 2014 10:12

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