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Light spectrum modifies the utilization pattern of energy sources in Pseudomonas sp. DR 5-09

Gharaie, Samareh and Vaas, Lea A. I. and Rosberg, Anna Karin and Windstam, Sofia and Karlsson, Maria and Bergstrand, Karl-Johan and Khalil, Sammar and Wohanka, Walter and Alsanius, Beatrix (2017). Light spectrum modifies the utilization pattern of energy sources in Pseudomonas sp. DR 5-09. PloS one. 12, 1-24
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Official URL: https://doi.org/10.1371/journal.pone.0189862

Abstract

Despite the overruling impact of light in the phyllosphere, little is known regarding the influence of light spectra on non-phototrophic bacteria colonizing the leaf surface. We developed an in vitro method to study phenotypic profile responses of bacterial pure cultures to different bands of the visible light spectrum using monochromatic (blue: 460 nm; red: 660 nm) and polychromatic (white: 350±990 nm) LEDs, by modification and optimization of a protocol for the Phenotype MicroArray™ technique (Biolog Inc., CA, USA). The new protocol revealed high reproducibility of substrate utilization under all conditions tested. Challenging the nonphototrophic bacterium Pseudomonas sp. DR 5±09 with white, blue, and red light demonstrated that all light treatments affected the respiratory profile differently, with blue LED having the most decisive impact on substrate utilization by impairing respiration of 140 substrates. The respiratory activity was decreased on 23 and 42 substrates under red and white LEDs, respectively, while utilization of one, 16, and 20 substrates increased in the presence of red, blue, and white LEDs, respectively. Interestingly, on four substrates contrasting utilization patterns were found when the bacterium was exposed to different light spectra. Although non-phototrophic bacteria do not rely directly on light as an energy source, Pseudomonas sp. DR 5±09 changed its respiratory activity on various substrates differently when exposed to different lights. Thus, ability to sense and distinguish between different wavelengths even within the visible light spectrum must exist, and leads to differential regulation of substrate usage. With these results, we hypothesize that different light spectra might be a hitherto neglected key stimulus for changes in microbial lifestyle and habits of substrate usage by non-phototrophic phyllospheric microbiota, and thus might essentially stratify leaf microbiota composition and diversity.

Authors/Creators:Gharaie, Samareh and Vaas, Lea A. I. and Rosberg, Anna Karin and Windstam, Sofia and Karlsson, Maria and Bergstrand, Karl-Johan and Khalil, Sammar and Wohanka, Walter and Alsanius, Beatrix
Title:Light spectrum modifies the utilization pattern of energy sources in Pseudomonas sp. DR 5-09
Series/Journal:PloS one (1932-6203)
Year of publishing :2017
Volume:12
Page range:1-24
Number of Pages:0
Publisher:Public Library of Science
Associated Programs and Other Stakeholders:Z - SLU - Libray > Odla mera
ISSN:1932-6203
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Agrovoc terms:microbial flora, light, Pseudomonas
Keywords:phyllosphere, non-phototrophic bacteria, Pseudomonas sp., light spectra, leaf microbiota
URN:NBN:urn:nbn:se:slu:epsilon-e-4684
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4684
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0189862
ID Code:15195
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Biosystems and Technology (from 130101)
(VH) > Department of Biosystems and Technology (from 130101)
Deposited By: SLUpub Connector
Deposited On:19 Jan 2018 11:50
Metadata Last Modified:20 Jan 2018 05:00

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