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Screening of intact yeasts and cell extracts to reduce Scrapie prions during biotransformation of food waste

Huyben, David and Boqvist, Sofia and Passoth, Volkmar and Renström, Lena H. M. and Allard Bengtsson, Ulrika and Andreoletti, Olivier and Kiessling, Anders and Lundh, Torbjörn and Vågsholm, Ivar (2018). Screening of intact yeasts and cell extracts to reduce Scrapie prions during biotransformation of food waste. Acta veterinaria Scandinavica. 60, 1-5
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Official URL: https://doi.org/10.1186/s13028-018-0363-y

Abstract

Yeasts can be used to convert organic food wastes to protein-rich animal feed in order to recapture nutrients. However, the reuse of animal-derived waste poses a risk for the transmission of infectious prions that can cause neurodegeneration and fatality in humans and animals. The aim of this study was to investigate the ability of yeasts to reduce prion activity during the biotransformation of waste substrates-thereby becoming a biosafety hurdle in such a circular food system. During pre-screening, 30 yeast isolates were spiked with Classical Scrapie prions and incubated for 72 h in casein substrate, as a waste substitute. Based on reduced Scrapie seeding activity, waste biotransformation and protease activities, intact cells and cell extracts of 10 yeasts were further tested. Prion analysis showed that five yeast species reduced Scrapie seeding activity by approximately 1 log10 or 90%. Cryptococcus laurentii showed the most potential to reduce prion activity since both intact and extracted cells reduced Scrapie by 1 log10 and achieved the highest protease activity. These results show that select forms of yeast can act as a prion hurdle during the biotransformation of waste. However, the limited ability of yeasts to reduce prion activity warrants caution as a sole barrier to transmission as higher log reductions are needed before using waste-cultured yeast in circular food systems.

Authors/Creators:Huyben, David and Boqvist, Sofia and Passoth, Volkmar and Renström, Lena H. M. and Allard Bengtsson, Ulrika and Andreoletti, Olivier and Kiessling, Anders and Lundh, Torbjörn and Vågsholm, Ivar
Title:Screening of intact yeasts and cell extracts to reduce Scrapie prions during biotransformation of food waste
Series/Journal:Acta veterinaria Scandinavica (0044-605X)
Year of publishing :2018
Volume:60
Page range:1-5
Number of Pages:5
Publisher:BioMed Central
ISSN:0044-605X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Cell and Molecular Biology
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Agrovoc terms:prions, food contamination, Scrapie agent, yeasts
Keywords:Food waste, Prions, Protease, Yeast
URN:NBN:urn:nbn:se:slu:epsilon-e-4814
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4814
ID Code:15361
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Department of Molecular Sciences
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
(VH) > Dept. of Animal Nutrition and Management
Deposited By: SLUpub Connector
Deposited On:14 Mar 2018 13:23
Metadata Last Modified:14 Mar 2018 13:23

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