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Agricultural factors affecting Fusarium communities in wheat kernels

Karlsson, Ida and Friberg, Hanna and Kolseth, Anna-Karin and Steinberg, Christian and Persson, Paula (2017). Agricultural factors affecting Fusarium communities in wheat kernels. International journal of food microbiology. 252, 53-60
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Official URL: http://dx.doi.org/10.1016/j.ijfoodmicro.2017.04.01...

Abstract

Fusarium head blight (FHB) is a devastating disease of cereals caused by Fusarium fungi. The disease is of great economic importance especially owing to reduced grain quality due to contamination by a range of mycotoxins produced by Fusarium. Disease control and prediction is difficult because of the many Fusarium species associated with FHB. Different species may respond differently to control methods and can have both competitive and synergistic interactions. Therefore, it is important to understand how agricultural practices affect Fusarium at the community level.Lower levels of Fusariwn mycotoxin contamination of organically produced cereals compared with conventionally produced have been reported, but the causes of these differences are not well understood. The aim of our study was to investigate the effect of agricultural factors on Fusarium abundance and community composition in different cropping systems. Winter wheat kernels were collected from 18 organically and conventionally cultivated fields in Sweden, paired based on their geographical distance and the wheat cultivar grown. We characterised the Fusarium community in harvested wheat kernels using 454 sequencing of translation elongation factor 1-alpha amplicons. In addition, we quantified Fusariwn spp. using real-time PCR to reveal differences in biomass between fields.We identified 12 Fusariwn operational taxonomic units (OTUs) with a median of 4.5 OTUs per field. Fusarium graminearum was the most abundant species, while F. avenaceum had the highest occurrence. The abundance of Fusariwn spp. ranged two orders of magnitude between fields. Two pairs of Fusariurt species co-occurred between fields: F. poae with F. tricinctwn and F. culmorwn with F. sporofrichoides. We could not detect any difference in Fusariwn communities between the organic and conventional systems. However, agricultural intensity, measured as the number of pesticide applications and the amount of nitrogen fertiliser applied, had an impact on Fusariwn communities, specifically increasing the abundance of F. tricinctwn. There were geographical differences in the Fusarium community composition where F. graminearwn was more abundant in the western part of Sweden. The application of amplicon sequencing provided a comprehensive view of the Fusarium community in cereals. This gives us better opportunities to understand the ecology of Fusarium spp., which is important in order to limit FHB and mycotoxin contamination in cereals.

Authors/Creators:Karlsson, Ida and Friberg, Hanna and Kolseth, Anna-Karin and Steinberg, Christian and Persson, Paula
Title:Agricultural factors affecting Fusarium communities in wheat kernels
Series/Journal:International journal of food microbiology (BIB9033352)
Year of publishing :2017
Volume:252
Page range:53-60
Number of Pages:8
Publisher:Elsevier
ISSN:0168-1605
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Agrovoc terms:cereal crops, plant diseases, fungi, Fusarium, wheat
Keywords:Triticum aestivum, Grain, Gibberella, High-throughput sequencing, Pathogen ecology
URN:NBN:urn:nbn:se:slu:epsilon-e-4910
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4910
ID Code:15445
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Crop Production Ecology
(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:04 Jun 2018 09:51
Metadata Last Modified:04 Jun 2018 09:51

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