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Investigating the Association Between the Caecal Microbiomes of Broilers and Campylobacter Burden

Sakaridis, Ioannis and Ellis, Richard John and Cawthraw, Shaun and Van Vliet, Arnoud H. M. and Stekel, Dov J. and Penell, Johanna and Chambers, Mark Andrew and La Ragione, Roberto M. and Cook, Alasdair J. (2018). Investigating the Association Between the Caecal Microbiomes of Broilers and Campylobacter Burden. Frontiers in Microbiology. 9, 1-9
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Abstract

One of the major transmission routes for the foodborne bacterial pathogen Campylobacter is undercooked poultry meat, contaminated from intestinal contents during processing. In broilers, Campylobacter can grow to very high densities in the caeca, and is often considered to be a commensal or an opportunistic pathogen in poultry. Reduction of caecal loads of Campylobacter may assist in lowering incidence rates of Campylobacter food poisoning. To achieve this, there needs to be a better understanding of the dynamics of Campylobacter colonization in its natural niche, and the effect of the local microbiome on colonization. Previous studies have shown that the microbiome differed between Campylobacter colonized and non-colonized chicken intestinal samples. To characterize the microbiome of Campylobacter-colonized broilers, caecal samples of 100 randomly selected birds from four farms were analyzed using amplified 16S rRNA gene sequences. Bacterial taxonomic analysis indicated that inter-farm variation was greater than intra-farm variation. The two most common bacterial groups were Bacteroidetes and Firmicutes which were present in all samples and constituted 29.7-63.5 and 30.2-59.8% of the bacteria present, respectively. Campylobacter was cultured from all samples, ranging from 2 to 9 log(10) CFU g(-1). There was no clear link between Campylobacter counts and Firmicutes, Bacteroidetes, or Tenericutes levels in the 16S rRNA operational taxonomic unit (OTU)-based analysis of the caecal microbiome, but samples with high Campylobacter counts (> 9 log CFU g(-1)) contained increased levels of Enterobacteriaceae. A decrease in Lactobacillus abundance in chicken caeca was also associated with high Campylobacter loads. The reported associations with Lactobacillus and Enterobacteriaceae match changes in the intestinal microbiome of chickens and mice previously reported for Campylobacter infection, and raises the question about temporality and causation; as to whether increases in Campylobacter loads create conditions adverse to Lactobacilli and/or beneficial to Enterobacteriaceae, or that changes in Lactobacilli and Enterobacteriaceae levels created conditions beneficial for Campylobacter colonization. If these changes can be controlled, this may open opportunities for modulation of chicken microbiota to reduce Campylobacter levels for improved food safety.

Authors/Creators:Sakaridis, Ioannis and Ellis, Richard John and Cawthraw, Shaun and Van Vliet, Arnoud H. M. and Stekel, Dov J. and Penell, Johanna and Chambers, Mark Andrew and La Ragione, Roberto M. and Cook, Alasdair J.
Title:Investigating the Association Between the Caecal Microbiomes of Broilers and Campylobacter Burden
Series/Journal:Frontiers in Microbiology (1664-302X)
Year of publishing :2018
Volume:9
Page range:1-9
Number of Pages:9
Publisher:Frontiers
ISSN:1664-302X
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
Agrovoc terms:broiler chickens, Campylobacter, microbial flora, intestinal flora
Keywords:caecal microbiomes, broilers, Campylobacter infections, sequencing, microbial community
URN:NBN:urn:nbn:se:slu:epsilon-e-4928
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4928
ID Code:15534
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:26 Jun 2018 09:03
Metadata Last Modified:26 Jun 2018 09:03

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