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Nitrogen uptake and assimilation during Norway spruce somatic embryogenesis

investigating the role of glutamine

Carlsson, Johanna M (2018). Nitrogen uptake and assimilation during Norway spruce somatic embryogenesis. Diss. (sammanfattning/summary) Umeå : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2018:43
ISBN 978-91-7760-226-2
eISBN 978-91-7760-227-9
[Doctoral thesis]

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Abstract

Sweden is currently experiencing shortage of Norway spruce (Picea abies) seeds due to e.g. irregular flowering and the recent year’s outbreaks of pests and pathogens destroying cones and seeds. Therefore, the forest sector is investigating alternative ways of propagating Norway spruce plants to secure Norway spruce regeneration and sustain the genetic diversity.

Somatic embryogenesis (SE) is a vegetative method that can be used for in vitro propagation of Norway spruce plants. Plants produced through the SE technique could potentially lead to; i) shorter time from recent tree breeding improvements to reforestation. ii) preserving genetic diversity in managed forests, while maintaining genetic gains from the tree breeding. SE can also be used as a mean to cryo-preserve valuable genetic material and to study embryo development. Unlike the seed embryo development, which is dependent on the surrounding tissue for nutrients, the entire SE development is undeniably dependent on the culture conditions; light, temperature, gaseous environment, media amendments and nutrient composition. I have used SE as a research tool to generate material for studying uptake, and utilization of carbon and nitrogen during Norway spruce embryo development.

My research has contributed new evidence that media supplied glutamine is essential for growth during the process of Norway spruce somatic embryogenesis. I found that media supplied with glutamine is important for maintaining the pool of free amino acids, the building blocks of e.g. DNA, proteins and chlorophyll. In addition, glutamine present in the growth medium assisted in alleviating stress-related metabolic pathways - the alanine aminotransferase pathway and the GABA shunt. Furthermore, the findings from my work can aid the on-going development for an industrial production system for Norway spruce plants through SE.

My research project has been a collaboration between Swedish university of agricultural science (SLU) and the forest company Sveaskog, specifically the seed and plant division Svenska Skogsplantor.

Authors/Creators:Carlsson, Johanna M
Title:Nitrogen uptake and assimilation during Norway spruce somatic embryogenesis
Subtitle:investigating the role of glutamine
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2018
Depositing date:31 August 2018
Volume:2018:43
Number of Pages:70
Papers/manuscripts:
NumberReferences
ICarlsson, J., Svennerstam, H., Moritz, T., Egertsdotter, U., Ganeteg, U.* (2017). Nitrogen uptake and assimilation in proliferating embryogenic cultures of Norway spruce - investigating the specific role of glutamine. PLoS ONE, vol 12 (8), e0181785.
IICarlsson, J., Egertsdotter, U., Ganeteg, U., Svennerstam, H.* (2018). Nitrogen utilization during germination of somatic embryos of Norway spruce - revealing the importance of supplied glutamine for nitrogen metabolism. (under revision)
IIICarlsson, J., Svennerstam, H.* The essential nature of exogenously supplied glutamine for Norway spruce somatic embryogenic cell culture. (Manuscript)
Place of Publication:Umeå
Publisher:Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-226-2
ISBN for electronic version:978-91-7760-227-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:F Plant production > F02 Plant propagation
F Plant production > F61 Plant physiology - Nutrition
F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Other Industrial Biotechnology
Agrovoc terms:somatic embryos, glutamin, Picea abies, in vitro production, nitrogen, genetic variation, vegative propagation
Keywords:Glutamine, Nitrogen, Norway spruce, Somatic embryogenesis
URN:NBN:urn:nbn:se:slu:epsilon-e-4979
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4979
ID Code:15636
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
External funders:Sveaskog
Deposited By: Johanna M Carlsson
Deposited On:05 Sep 2018 06:37
Metadata Last Modified:16 Nov 2018 09:29

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