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Detection and characterisation of novel vector-borne viruses in Mozambique

Cholleti, Harindranath (2018). Detection and characterisation of novel vector-borne viruses in Mozambique. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2018:42
ISBN 978-91-7760-224-8
eISBN 978-91-7760-225-5
[Doctoral thesis]

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Abstract

Arthropod vectors carry a wide variety of viruses that can cause vector-borne infectious diseases that affect the health of both humans and animals. The vector-borne viruses present in nature show high diversity and can infect a broad range of hosts. Moreover, some of these viruses may be transmitted to new hosts in the future and possibly become pathogenic and/or zoonotic.

This thesis describes the detection and characterisation of novel vector-borne viruses circulating in arthropod vectors from an understudied area, the Zambezi Valley of Mozambique, using a viral metagenomic approach. A combination of sequence-independent amplification, high-throughput sequencing and different bioinformatic tools were used to investigate the viromes of mosquitos and ticks in this area. The results of this study resulted in the identification of a broad range of viruses belonging to several viral families, including Flaviviridae, Rhabdoviridae, Iflaviridae, Picornaviridae, and Dicistroviridae, as well as a number of unclassified RNA viruses that were present in Culex and Mansonia spp. mosquitoes. The full-length genome of a novel flavivirus from Mansonia was characterised, and an analysis of conserved domains showed that it belongs to the insect-specific flaviviruses and is closely related to Nakiwogo virus. Further, a near full-length genome of a highly divergent picorna-like virus from Culex mosquitoes was characterised, and the phylogenetic analysis showed that the novel picorna-like virus clustered with members of iflaviruses, confirming that it belongs to the Iflaviridae viral family in the order Picornavirales. Analysis of the Rhipicephalus tick virome identified a number of reads classified as being in the Orthomyxoviridae family. The assembled contigs showed an amino acid identity (32-52%) towards known viruses in the genus Quaranjavirus.

Overall, viral metagenomics was successfully used to detect and characterise the viromes of important vectors (mosquitoes and ticks) from Mozambique. All the viral sequences identified from this study were divergent from previously known viruses and potentially represent novel viruses that are circulating in the Zambezi Valley of Mozambique. These results constitute the basis for further studies on viruses that are circulating in vectors and on the evolution, viral maintenance and transmission of pathogenic arboviruses.

Authors/Creators:Cholleti, Harindranath
Title:Detection and characterisation of novel vector-borne viruses in Mozambique
Series/Journal:Acta Universitatis agriculturae Sueciae (1652-6880)
Year of publishing :2018
Depositing date:10 September 2018
Volume:2018:42
Number of Pages:64
Papers/manuscripts:
NumberReferences
ICholleti, H.*, Hayer, J., Abilio, A.P., Mulandane, F.C., Verner-Carlsson, J., Falk, K.I., Fafetine, J.M., Berg, M. & Blomstrom, A.L. (2016). Discovery of Novel Viruses in Mosquitoes from the Zambezi Valley of Mozambique. PLoS ONE, 11(9), p. e0162751.
IICholleti, H.*, Hayer, J., Fafetine, J., Berg, M. & Blomstrom, A.L. (2018). Genetic characterisation of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique. Virology Journal, 15(1), p. 71.
IIICholleti, H.*, Hayer, J., Mulandane, F.C., Falk, K., Fafetine, J., Berg, M. & Blomstrom, A.L. (2018). Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique. Infection Ecology & Epidemiology, 8(1), p. 1478585.
Place of Publication:Uppsala
Publisher:Department of Biomedical Sciences and Veterinary Public Health Uppsala
ISBN for printed version:978-91-7760-224-8
ISBN for electronic version:978-91-7760-225-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X30 Life sciences
Subjects:Obsolete subject words > VETERINARY MEDICINE > Microbiology and immunology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
Agrovoc terms:vectorborne diseases, viruses, Arthropoda, genomics, natural environmnent, sampling, Culicidae, Metastigmata, Mozambique
Keywords:Vector-borne viruses, Viral metagenomics, Insect-specific flaviviruses, RNA viruses, Mozambique, Mosquitoes, Ticks, Picorna-like virus, Quaranjavirus
URN:NBN:urn:nbn:se:slu:epsilon-e-4990
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-4990
ID Code:15651
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: Harindranath Cholleti
Deposited On:11 Sep 2018 08:44
Metadata Last Modified:11 Sep 2018 08:44

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