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Assessing Cu impacts on freshwater diatoms: biochemical and metabolomic responses of Tabellaria flocculosa (Roth) Kutzing

Goncalves, Sara and Kahlert, Maria and Almeida, Salomé F.P. and Figueira, Etelvina (2018). Assessing Cu impacts on freshwater diatoms: biochemical and metabolomic responses of Tabellaria flocculosa (Roth) Kutzing. Science of the total environment. 625 , 1234-1246
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Abstract

Metals are a recognised threat to aquatic organisms but the impact of metals such as copper (Cu) on benthic freshwater diatoms is poorly understood, even if diatoms are commonly used as water quality indicators. Our study aimed to elucidate the cellular targets of Cu toxicity and the mechanisms cells resort to counteract toxicity and to increase tolerance to Cu. A concerted approach analysing the biochemical, physiological and metabolome alterations in diatom cells was conducted by exposing the freshwater diatom Tabellaria flocattosa to 0, 0.3, 6 and 10 mu g Cu/L. Cu was already toxic to T. flocculosa at concentrations common in environments and which are not usually considered to be contaminated (0.3 mu g Cu/L). Under Cu impact, the metabolome of T. flocculosa changed significantly, especially at high concentrations (6 and 10 mu g Cu/L). Cu toxicity was counteracted by increasing extracellular immobilization (EPS, frustulins), antioxidant (SOD, CAT) and detoxifying (GSTs) enzymes activity and low molecular weight antioxidants (GSH). These mechanisms were fuelled by higher energy production (increased ETS activity). At the highest Cu concentration (10 mu g/L), these processes were specially enhanced in an attempt to restrain the oxidative stress generated by high intracellular Cu concentrations. However, these mechanisms were not able to fully protect cells, and damage in membranes and proteins increased. Moreover, the decrease of hydroxylamine and unsaturated fatty acids and the increase of saturated fatty acids, 2-palmiloylglycerol, glycerol and diterpenoid compounds should be tested as new specific markers of Cu toxicity in future studies. This information can support the prediction of diatom behaviour in different Cu contamination levels, including highly impacted environments, such as mining scenarios, and may assist in environmental risk assessment policies and restoration programs. (C) 2018 Elsevier B.V. All rights reserved.

Authors/Creators:Goncalves, Sara and Kahlert, Maria and Almeida, Salomé F.P. and Figueira, Etelvina
Title:Assessing Cu impacts on freshwater diatoms: biochemical and metabolomic responses of Tabellaria flocculosa (Roth) Kutzing
Series/Journal:Science of the total environment (0048-9697)
Year of publishing :2018
Volume:625
Page range:1234-1246
Number of Pages:13
Publisher:Elsevier
Associated Programs and Other Stakeholders:SLU - Environmental assessment > Programme Non-Toxic Environment
ISSN:0048-9697
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 105 Earth and Related Environmental Sciences > Environmental Sciences (social aspects to be 507)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Fish and Aquacultural Science
Keywords:Copper, Freshwater diatoms, Tabellaria flocculosa, Physiological indicators, Metabolomics
URN:NBN:urn:nbn:se:slu:epsilon-e-5199
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5199
Additional ID:
Type of IDID
DOI10.1016/j.scitotenv.2017.12.320
Web of Science (WoS)000426356600124
ID Code:15797
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
Deposited By: SLUpub Connector
Deposited On:31 Jan 2019 07:55
Metadata Last Modified:13 Jan 2020 00:15

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