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Refinement of mouse husbandry for improved animal welfare and research quality

Marques, Joana M. (2007). Refinement of mouse husbandry for improved animal welfare and research quality. Diss. (sammanfattning/summary) SLU : Sveriges lantbruksuniv., Acta Universitatis agriculturae Sueciae, 1652-6880 ; 2007:104
ISBN 978-91-859-1303-9
[Doctoral thesis]

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Abstract

Following advances in genetic engineering, mice are today the most commonly used mammal in research. This thesis addresses two issues: the impact on animal welfare and research quality by environmental enrichment and the characterization of genetically modified mice. In the first paper, the effects of environmental enrichment before weaning were studied. No alterations were found at age 4 weeks, but after 8 weeks of environmental enrichment the home cage activity had decreased. In the second paper, a battery of behavioural tests was used to characterize a spontaneous mouse mutant, the leaner heterozygous. This mutant showed cognitive and motor impairment. This finding was followed up, in the third paper, by housing the leaner heterozygous and wild type mice in cages with three different degrees of environmental complexity. In Morris watermaze tests at 6, 12 and 20 months of age, environmental enrichment was found to increase learning and memory capacity in mice of both genotypes. In paper IV, a characterization routine is presented for pre-weaning monitoring of mutant mice. Individual pups were scored for several development parameters on postnatal days 1, 3, 7 and 14. At weaning (day 19 to 21) a clinical examination and the novel cage test for emotional reactivity were carried out. The protocol was tested in several C57BL/6, BALB/C, 129S6 and B6CBAF1 litters. The characterization routine was efficient in detecting differences between genotypes in weight gain, physical condition at weaning, reaction to handling and emotional reactivity. In paper V, C57BL/6, 129S6 and B6CBAF1 mice were used. The mice were tested in the novel cage (at weaning), the open Field test (at 5 weeks), and from 9 weeks of age in the elevated plus-maze, the concentric square field and the rat exposure test. Results obtained with the novel cage test at weaning were largely consistent with those obtained in the established tests later in life. In conclusion, this thesis shows that cage enrichment decreases the cognitive deficits in the heterozygous leaner mouse. The new protocol for pre-weaning monitoring can be used to detect deviations in early development and emotional reactivity in a number of commonly used mice genotypes.

Authors/Creators:Marques, Joana M.
Title:Refinement of mouse husbandry for improved animal welfare and research quality
Year of publishing :October 2007
Volume:2007:104
Number of Pages:52
Papers/manuscripts:
NumberReferences
ALLI. Marques, JM and Olsson, IAS. 2007. The effect of preweaning and postweaning environment on the behaviour of the laboratory mouse (Mus musculus). Laboratory Animals 41(1): 92 – 102. II. Alonso, I, Marques, JM, Sousa, N, Sequeiros, J, Olsson, VV.IAS and Silveira, I. Motor and cognitive impairments in the leaner heterozygous, a Cav2.1 voltage-gated Ca2+ mutant. Neurobiology of Aging (2007), doi: 10.1016/j.neurobiolaging.2007.04.005. In Press. III. Marques, JM, Alonso, I, Silveira, I, Santos, C and Olsson IAS. The effect of environmental enrichment on the cognitive impairment of the leaner heterozygous. Submitted. IV. Marques, JM, Ögren, SO, Augustsson, H and Dahlborn, K. A characterization routine for improved animal welfare in pre-weaning mice. Manuscript V. Marques, JM, Olsson, IAS, Ögren SO and Dahlborn, K. Evaluation of exploration and risk assessment in pre-weaning mice using the novel cage test. Physiology & Behavior (2007), doi: 10.1016/j.physbeh.2007.08.006. In Press.
Place of Publication:SLU
ISBN for printed version:978-91-859-1303-9
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Full Text Status:Public
Agrovoc terms:mice, genetic engineering, animal husbandry, animal welfare
Keywords:Refinement, husbandry, phenotyping, environmental enrichment, welfare, mice, emotional reactivity
URN:NBN:urn:nbn:se:slu:epsilon-1823
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-1823
ID Code:1581
Department:(VH) > Dept. of Clinical Sciences
Deposited By: Joana Marques
Deposited On:08 Oct 2007 00:00
Metadata Last Modified:02 Dec 2014 10:12

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