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Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation

Hallberg, Ida and Kjellgren, Jacklin and Persson, Sara and Örn, Stefan and Sjunnesson, Ylva (2019). Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation. Reproductive toxicology. 84, 1-8
[Journal article]

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Official URL: https://doi.org/10.1016/j.reprotox.2018.11.005

Abstract

Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 μg mL−1 and 0.1 μg mL−1), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 μg mL−1 were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 μg mL−1 of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p < 0.05), an effect not observed at 0.1 μg mL−1. However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 μg mL−1, suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro.

Authors/Creators:Hallberg, Ida and Kjellgren, Jacklin and Persson, Sara and Örn, Stefan and Sjunnesson, Ylva
Title:Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation
Series/Journal:Reproductive toxicology (0890-6238)
Year of publishing :2019
Volume:84
Page range:1-8
Number of Pages:8
Publisher:Elsevier
ISSN:0890-6238
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Full Text Status:Restricted
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Other Veterinary Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Bovine, IVP, PFNA, Lipid droplet, Image analysis, Developmental toxicology, Endocrine disruption
URN:NBN:urn:nbn:se:slu:epsilon-e-5171
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5171
Additional ID:
Type of IDID
DOI10.1016/j.reprotox.2018.11.005
ID Code:15850
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
External funders:FORMAS
Deposited By: SLUpub Connector
Deposited On:18 Jan 2019 09:22
Metadata Last Modified:23 Jan 2019 08:40

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