Hallberg, Ida and Kjellgren, Jacklin and Persson, Sara and Örn, Stefan and Sjunnesson, Ylva
(2019).
Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation.
Reproductive toxicology. 84
, 1-8
[Journal article]
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PDF
- Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives. 881kB |
Official URL: https://doi.org/10.1016/j.reprotox.2018.11.005
Abstract
Perfluorononanoic acid (PFNA) is one of the perfluoroalkyl acids present in human tissues. In this study, effects on early embryo development after PFNA exposure were investigated using the bovine in vitro production system. Oocytes were exposed to PFNA during maturation in vitro (10 μg mL−1 and 0.1 μg mL−1), and then fertilized and cultured in parallel with control groups. Developmental parameters (cleavage, blastocyst formation) were followed and embryo quality evaluated (stage, grade). Embryos developed after exposure to 0.1 μg mL−1 were stained to distinguish nuclei, active mitochondria and neutral lipids. 10 μg mL−1 of PFNA had a severe negative effect on blastocyst formation (OR: 0.27 p < 0.05), an effect not observed at 0.1 μg mL−1. However, lipid droplet distribution was significantly altered in embryos exposed to 0.1 μg mL−1, suggesting a disturbance of lipid metabolism after exposure to sublethal levels of PFNA during oocyte maturation in vitro.
Authors/Creators: | Hallberg, Ida and Kjellgren, Jacklin and Persson, Sara and Örn, Stefan and Sjunnesson, Ylva | ||||
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Title: | Perfluorononanoic acid (PFNA) alters lipid accumulation in bovine blastocysts after oocyte exposure during in vitro maturation | ||||
Series Name/Journal: | Reproductive toxicology | ||||
Year of publishing : | 2019 | ||||
Volume: | 84 | ||||
Page range: | 1-8 | ||||
Number of Pages: | 8 | ||||
Publisher: | Elsevier | ||||
ISSN: | 0890-6238 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Accepted version | ||||
Copyright: | Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Medical Bioscience (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Other Veterinary Science (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science | ||||
Keywords: | Bovine, IVP, PFNA, Lipid droplet, Image analysis, Developmental toxicology, Endocrine disruption | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-5171 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5171 | ||||
Additional ID: |
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ID Code: | 15850 | ||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||
Department: | (VH) > Dept. of Clinical Sciences (VH) > Dept. of Biomedical Sciences and Veterinary Public Health | ||||
External funders: | FORMAS | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 18 Jan 2019 09:22 | ||||
Metadata Last Modified: | 29 Nov 2019 00:15 |
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