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Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique

Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie (2018). Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique. Infection ecology & epidemiology. 8, 1-8
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Official URL: https://doi.org/10.1080/20008686.2018.1478585

Abstract

Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, CrimeanCongo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique.
Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences.
Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA.
Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus.

Authors/Creators:Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie
Title:Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique
Series/Journal:Infection ecology & epidemiology (BIB12539748)
Year of publishing :2018
Volume:8
Page range:1-8
Number of Pages:8
Publisher:Taylor & Francis
ISSN:2000-8686
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Public Health, Global Health, Social Medicine and Epidemiology
Keywords:ticks, arthropods, Rhipicephalus, quaranjavirus, viral metagenomics, Mozambique
URN:NBN:urn:nbn:se:slu:epsilon-e-5222
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5222
Additional ID:
Type of IDID
DOI10.1080/20008686.2018.1478585
ID Code:15903
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(NL, NJ) > Institutionen för husdjursgenetik (fr.o.m. 960101)
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:12 Feb 2019 12:57
Metadata Last Modified:12 Feb 2019 12:57

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