Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie
(2018).
Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique.
Infection ecology & epidemiology. 8
, 1-8
[Research article]
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Official URL: https://doi.org/10.1080/20008686.2018.1478585
Abstract
Background: Ticks are primary vectors for many well-known disease-causing agents that affect human and animal populations globally such as tick-borne encephalitis, CrimeanCongo hemorrhagic fever and African swine fever. In this study, viral metagenomics was used to identify what viruses are present in Rhipicephalus spp. ticks collected in the Zambezi Valley of Mozambique.
Methods: The RNA was amplified with sequence-independent single primer amplification (SISPA) and high-throughput sequencing was performed on the Ion Torrent platform. The generated sequences were subjected to quality check and classfied by BLAST. CodonCode aligner and SeqMan were used to assemble the sequences.
Results: The majority of viral sequences showed closest sequence identity to the Orthomyxoviridae family, although viruses similar to the Parvoviridae and Coronaviridae were also identified. Nearly complete sequences of five orthomyxoviral segments (HA, NP, PB1, PB2, and PA) were obtained and these showed an amino acid identity of 32–52% to known quaranjaviruses. The sequences were most closely related to the Wellfleet Bay virus, detected and isolated from common eider during a mortality event in the USA.
Conclusions: In summary, this study has identified a highly divergent virus with in the Orthomyxoviridae family associated with Rhipicephalus ticks from Mozambique. Further genetic and biological studies are needed in order to investigate potential pathogenesis of the identified orthomyxovirus.
Authors/Creators: | Cholleti, Harindranath and Hayer, Juliette and Mulandane, Fernando Chanisso and Falk, Kerstin and Fafetine, José and Berg, Mikael and Blomström, Anne-Lie | ||||
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Title: | Viral metagenomics reveals the presence of highly divergent quaranjavirus in Rhipicephalus ticks from Mozambique | ||||
Series Name/Journal: | Infection ecology & epidemiology | ||||
Year of publishing : | 2018 | ||||
Volume: | 8 | ||||
Page range: | 1-8 | ||||
Number of Pages: | 8 | ||||
Publisher: | Taylor & Francis | ||||
ISSN: | 2000-8686 | ||||
Language: | English | ||||
Publication Type: | Research article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Pathobiology (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 303 Health Sciences > Public Health, Global Health, Social Medicine and Epidemiology | ||||
Keywords: | ticks, arthropods, Rhipicephalus, quaranjavirus, viral metagenomics, Mozambique | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-e-5222 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5222 | ||||
Additional ID: |
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ID Code: | 15903 | ||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap VH - Faculty of Veterinary Medicine and Animal Science | ||||
Department: | (NL, NJ) > Institutionen för husdjursgenetik (fr.o.m. 960101) (VH) > Dept. of Biomedical Sciences and Veterinary Public Health | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 12 Feb 2019 12:57 | ||||
Metadata Last Modified: | 12 Feb 2019 12:57 |
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