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Sperm quality during storage is not affected by the presence of antibiotics in EquiPlus semen extender but is improved by single layer centrifugation

Al-Kass, Ziyad and Spergser, Joachim and Aurich, Christine and Kuhl, Juliane and Schmidt, Kathrin and Johannisson, Anders and Morrell, Jane (2017). Sperm quality during storage is not affected by the presence of antibiotics in EquiPlus semen extender but is improved by single layer centrifugation. Antibiotics. 7 , 1-13
[Journal article]

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Official URL: http://dx.doi.org/10.3390/antibiotics7010001

Abstract

Contamination of semen with bacteria arises during semen collection and handling. This bacterial contamination is typically controlled by adding antibiotics to semen extenders but intensive usage of antibiotics can lead to the development of bacterial resistance and may be detrimental to sperm quality. The objective of this study was to determine the effects of antibiotics in a semen extender on sperm quality and to investigate the effects of removal of bacteria by modified Single Layer Centrifugation (MSLC) through a colloid. Semen was collected from six adult pony stallions (three ejaculates per male). Aliquots of extended semen were used for MSLC with Equicoll, resulting in four treatment groups: control and MSLC in extender with antibiotics (CA and SA, respectively); control and MSLC in extender without antibiotics (CW and SW, respectively). Sperm motility, membrane integrity, mitochondrial membrane potential and chromatin integrity were evaluated daily by computer-assisted sperm analysis (CASA) and flow cytometry. There were no differences in sperm quality between CA and CW, or between SA and SW, although progressive motility was negatively correlated to total bacterial counts at 0 h. However, MSLC groups showed higher mean total motility (P < 0.001), progressive motility (P < 0.05), membrane integrity (P < 0.0001) and mitochondrial membrane potential (P < 0.05), as well as better chromatin integrity (P < 0.05), than controls. Sperm quality remained higher in the MSLC groups than controls throughout storage. These results indicate that sperm quality was not adversely affected by the presence of antibiotics but was improved considerably by MSLC.

Authors/Creators:Al-Kass, Ziyad and Spergser, Joachim and Aurich, Christine and Kuhl, Juliane and Schmidt, Kathrin and Johannisson, Anders and Morrell, Jane
Title:Sperm quality during storage is not affected by the presence of antibiotics in EquiPlus semen extender but is improved by single layer centrifugation
Year of publishing :2017
Volume:7
Page range:1-13
Number of Pages:13
Publisher:MDPI
ISSN:2079-6382
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:pony stallions, bacteria, semen evaluation
URN:NBN:urn:nbn:se:slu:epsilon-e-5316
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-e-5316
Additional ID:
Type of IDID
DOIUNSPECIFIED
ID Code:16025
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:01 Apr 2019 05:42
Metadata Last Modified:01 Apr 2019 05:42

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