Al-Essawe, Essraa M
(2019).
Equine seminal plasma: its role in protecting stallion spermatozoa during cryopreservation.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2019:22
ISBN 978-91-7760-362-7
eISBN 978-91-7760-363-4
[Doctoral thesis]
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PDF
864kB |
Abstract
There is considerable variability in the ability of spermatozoa from different stallions to withstand the freezing process. As a result, stallions are usually categorized as good or bad freezers. In the equine industry, the demand for frozen semen for artificial insemination is increasing, resulting in a need for optimising cryopreservation techniques. Previously the removal of seminal plasma (SP) prior to cryopreservation was shown to improve post-thaw sperm quality, whereas the effect of adding SP on spermatozoa varied among studies. Single Layer Centrifugation (SLC) is a technique that has been used to select the most robust spermatozoa from the ejaculate and prolong their viability, as well as separating them from SP. The aims of this thesis were: 1) to study the effect of combining SLC with addition of SP from stallions of known freezability on sperm cryosurvival, including their ability to bind to heterologous oocytes; 2) To explore the differences in the major protein groups in SP between the breeding and non-breeding seasons in relation to sperm quality. Selecting robust spermatozoa using SLC prior to freezing improved post-thaw sperm quality, whereas addition of pooled SP from good or bad freezers to the selected sperm samples did not have an additional beneficial effect. Sperm quality was adversely impaired after restoring pooled SP to SLC-selected spermatozoa after thawing, independently of whether the SP came from a good or a bad freezer stallion, suggesting an inability of SP to repair stallion sperm cryoinjuries in the presence of an egg yolk-containing extender. Sperm binding affinity to the zona pellucida (ZP) of bovine oocytes was reduced after adding SP from good freezer stallions prior to cryopreservation. However, when SP was added after thawing, the mean number of bound spermatozoa was higher for the group treated with SP from good freezers than for the group treated with SP from bad freezers. Minor differences in protein composition were observed between seasons as well as between good and bad freezers. In the nonbreeding season there was a higher content of heparin-binding proteins and also of a subset of non-heparin binding proteins compared to the breeding season; these proteins may be linked to fertility. Sperm quality did not decline during the non-breeding season and SP-testosterone levels did not vary between seasons. Customization of the cryopreservation protocol for individual stallions, including studying the effect of adding SP from individual stallions, would be required to optimize the effect on sperm cryosurvival. Global proteome investigation of SP proteins could help to identify cryosurvival biomarkers for stallion spermatozoa.
Authors/Creators: | Al-Essawe, Essraa M | ||||||||||
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Title: | Equine seminal plasma: its role in protecting stallion spermatozoa during cryopreservation | ||||||||||
Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||||||||
Year of publishing : | 2019 | ||||||||||
Number: | 2019:22 | ||||||||||
Number of Pages: | 90 | ||||||||||
Papers/manuscripts: |
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Place of Publication: | Uppsala | ||||||||||
Publisher: | Department of Clinical Sciences, Swedish University of Agricultural Sciences | ||||||||||
ISBN for printed version: | 978-91-7760-362-7 | ||||||||||
ISBN for electronic version: | 978-91-7760-363-4 | ||||||||||
ISSN: | 1652-6880 | ||||||||||
Language: | English | ||||||||||
Publication Type: | Doctoral thesis | ||||||||||
Article category: | Other scientific | ||||||||||
Full Text Status: | Public | ||||||||||
Agris subject categories.: | L Animal production > L53 Animal physiology - Reproduction | ||||||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science | ||||||||||
Keywords: | equine, seminal plasma, spermatozoa, freezability, good freezer, bad freezer, zona pellucida, ßI, cryoinjuries, ROS | ||||||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-99414 | ||||||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-99414 | ||||||||||
ID Code: | 16053 | ||||||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||||||
Department: | (VH) > Dept. of Clinical Sciences | ||||||||||
Deposited By: | SLUpub Connector | ||||||||||
Deposited On: | 12 Apr 2019 07:53 | ||||||||||
Metadata Last Modified: | 17 Apr 2019 09:29 |
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