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Structural studies of hyaluronan hydrogels

Wende, Frida J. (2019). Structural studies of hyaluronan hydrogels. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2019:30
ISBN 978-91-7760-378-8
eISBN 978-91-7760-379-5
[Doctoral thesis]

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Abstract

Hydrogels consist of cross-linked polymer networks with the ability to absorb large amounts of water. In this thesis, the structures of hydrogels consisting of hyaluronic acid (HA) cross-linked by 1,4-butanediol diglycidyl ether (BDDE) have been investigated. Hyaluronic acid is a naturally occurring polysaccharide that is abundant in the extracellular matrix of all vertebrates. Due to high viscoelasticity and biocompatibility, hyaluronic acid based hydrogels have many biomedical applications.

Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS), which are among the most important analytical techniques for analysis of organic compounds and biomacromolecules, were used for analysis of structural properties of HA-BDDE hydrogels.

NMR- and MS-based methods were developed to determine the positions of substitution of the cross-linker on HA in fragments obtained from enzymatic degradation of HA-BDDE hydrogels. One-dimensional proton and carbon NMR methods were then further developed for quantitative analysis of substitution positions, degree of modification and degree of cross-linking. No chromatographic separation step was needed.

Desulphated chondroitin has a primary structure that only differs from that of HA by the configuration at C4 of the aminoglycan. The reactivity of desulphated chondroitin towards BDDE as well as alkali-induced changes in chemical shifts were determined and compared to that of hyaluronic acid. Using NMR spectroscopy of hydroxyl protons, hydrogen bonding in the two polymers were compared.

Water is the most abundant component of the studied hydrogels. Using NMR spectroscopy, the relaxation and diffusion behaviour of water protons in native HA solutions and cross-linked HA hydrogels with different physicochemical properties were studied. The transverse relaxation, which is dominated by chemical exchange, could be correlated to polysaccharide mobility.

Authors/Creators:Wende, Frida J.
Title:Structural studies of hyaluronan hydrogels
Series/Journal:Acta Universitatis Agriculturae Sueciae (1652-6880)
Year of publishing :2019
Volume:2019:30
Number:2019:30
Number of Pages:85
Papers/manuscripts:
NumberReferences
IFrida J. Wende, Suresh Gohil, Hotan Mojarradi, Thibaud Gerfaud, Lars I. Nord, Anders Karlsson, Jean-Guy Boiteau, Anne Helander Kenne, Corine Sandström.(2016). Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods.Carbohydrate Polymers,136,1348-1357.
IIFrida J. Wende, Suresh Gohil, Lars I. Nord, Anne Helander Kenne, Corine Sandström.(2017). 1D NMR methods for determination of degree of cross-linking and BDDE substitution positions in HA hydrogels. Carbohydrate Polymers,157,1525-1530.
IIIFrida J. Wende, Suresh Gohil, Lars I. Nord, Anders Karlsson, Anne Helander Kenne, Corine Sandström.(2019). Insights on the reactivity of chondroitin and hyaluronan toward 1,4-butanediol diglycidyl ether. International Journal of Biological Macromolecules, 131, 812-820.
IVFrida J. Wende, Gustav Nestor, Åke Öhrlund, Corine Sandström. Relaxation and diffusion of waterprotonsin BDDE cross-linked hyaluronic acid hydrogelsinvestigatedby NMR spectroscopy – comparison with physicochemical properties (Manuscript)
Place of Publication:Uppsala
Publisher:Department of Molecular Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-378-8
ISBN for electronic version:978-91-7760-379-5
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Full Text Status:Public
Agris subject categories.:X Agricola extesions > X50 Chemistry
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry
(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Polymer Chemistry
(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Organic Chemistry
Keywords:hyaluronan, NMR spectroscopy, mass spectrometry, polysaccharide, glycosaminoglycan, cross-linking, hydrogel
URN:NBN:urn:nbn:se:slu:epsilon-p-99723
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-99723
ID Code:16109
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:02 May 2019 08:05
Metadata Last Modified:02 May 2019 08:05

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