Home About Browse Search
Svenska


Evaluation of entomopathogenic fungi Metarhizium anisopliae against dengue virus mosquitoes Aedes aegypti (Diptera: Culicidae)

Jemberie, Wondmeneh and Hill, Sharon and Rännbäck, Linda-Marie and Ondiaka, Sopher and Raja, Nagappan and Ignell, Rickard (2018). Evaluation of entomopathogenic fungi Metarhizium anisopliae against dengue virus mosquitoes Aedes aegypti (Diptera: Culicidae). Entomon. 43 , 7-18
[Journal article]

[img] PDF (Published here with kind permission from the Editor.) - Published Version
3MB

Abstract

In this study, the bio-potential of the entomopathogenic fungus Metarhizium anisopliae was tested against Aedes aegypti under laboratory conditions. The study includes the analysis of the attractive response, survival and fecundity rate of non-blood and blood fed female mosquitoes exposed to the volatiles of two M. anisopliae strains. The attractive response was analysed using a two-choice behavioural bioassay, with three different sizes of dry spore plates (full, 1/4 and 1/16 plates). The survival and fecundity bioassay was conducted simultaneously in plastic pots. Log-rank survival curve analysis was used for statistical comparisons of the attractive response, survival and fecundity. Non-blood and blood fed mosquitoes were highly attracted to M. anisopliae30 volatiles compared with that of the M. anisopliae-131 strain. Moreover, attraction was dependent on the size of the dry spore plate. Survival was completely abolished in unfed mosquitoes 5 and 6 days after treatment with 109 spores/mL of M. anisopliae-30 and M. anisopliae -131, respectively, whereas almost 80% of untreated unfed females survived more than 28 days. Survival in blood fed mosquitoes treated with same dose of M. anisopliae-30 and M. anisopliae-131was abolished after 6 and 7 days, respectively, while over 80% of untreated blood fed females survived more than 28 days in the controls. Mean number of eggs laid by blood fed mosquitoes treated with 109 spores/mL of M. anisopliae-131 was 26 ± 3 compared to control (67 ± 4). However for M. anisopliae-30, 19 ± 3 eggs were laid compared to control 72 ± 5 eggs. This study concludes that both the strains of M. anisopliae reduce egg laying capacity and survival rate in Ae. aegypti. As such, these strains can be useful for the development of mycoinsecticides for the control of the dengue fever vector mosquito, Ae. aegypti

Authors/Creators:Jemberie, Wondmeneh and Hill, Sharon and Rännbäck, Linda-Marie and Ondiaka, Sopher and Raja, Nagappan and Ignell, Rickard
Title:Evaluation of entomopathogenic fungi Metarhizium anisopliae against dengue virus mosquitoes Aedes aegypti (Diptera: Culicidae)
Year of publishing :2018
Volume:43
Page range:7-18
Number of Pages:12
Publisher:Association for Advancement of Entomology
Associated Programs and Other Stakeholders:Z - SLU - Libray > Odla mera
ISSN:0377-9335
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Behavioural Sciences Biology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology
Keywords:Aedes aegypti, attractive response, fecundity rate, Metarhizium anisopliae, survival rate, vector control
URN:NBN:urn:nbn:se:slu:epsilon-p-100435
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-100435
ID Code:16203
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:09 Jul 2019 06:01
Metadata Last Modified:10 Jul 2019 04:01

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits