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Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population

Ohlsson, Jonas and Hallingbäck, Henrik and Jebrane, Mohamed and Harman-Ware, Anne E. and Shollenberger, Todd and R. Decker, Stephen and Sandgren, Mats and Rönnberg Wästljung, Ann-Christin (2019). Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population. Biotechnology for Biofuels. 12 :135 , 1-12
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Abstract

Background: Salix spp. are high-productivity crops potentially used for lignocellulosic biofuels such as bioethanol. In general, pretreatment is needed to facilitate the enzymatic depolymerization process. Biomass resistance to degradation, i.e., biomass recalcitrance, is a trait which can be assessed by measuring the sugar released after combined pretreatment and enzymatic hydrolysis. We have examined genetic parameters of enzymatic sugar release and other traits related to biorefnery use in a population of 286 natural Salix viminalis clones. Furthermore, we have evaluated phenotypic and genetic correlations between these traits and performed a genomewide association mapping analysis using a set of 19,411 markers.
Results: Sugar release (glucose and xylose) after pretreatment and enzymatic saccharifcation proved highly variable with large genetic and phenotypic variations, and chip heritability estimates (h2 ) of 0.23–0.29. Lignin syringyl/guaiacyl (S/G) ratio and wood density were the most heritable traits (h2=0.42 and 0.59, respectively). Sugar release traits were positively correlated, phenotypically and genetically, with biomass yield and lignin S/G ratio. Association mapping revealed seven marker–trait associations below a suggestive signifcance threshold, including one marker associated with glucose release.
Conclusions: We identifed lignin S/G ratio and shoot diameter as heritable traits that could be relatively easily evaluated by breeders, making them suitable proxy traits for developing low-recalcitrance varieties. One marker below the suggestive threshold for marker associations was identifed for sugar release, meriting further investigation while also highlighting the difculties in employing genomewide association mapping for complex traits

Authors/Creators:Ohlsson, Jonas and Hallingbäck, Henrik and Jebrane, Mohamed and Harman-Ware, Anne E. and Shollenberger, Todd and R. Decker, Stephen and Sandgren, Mats and Rönnberg Wästljung, Ann-Christin
Title:Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population
Year of publishing :2019
Volume:12
Number:135
Page range:1-12
Number of Pages:0
Publisher:BMC
ISSN:1754-6834
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Wood Science
Keywords:Salix viminalis, Bioenergy crops, Lignocellulosic biofuels, Genetic parameters, Genomewide association study, Enzymatic saccharifcation, Biomass recalcitrance, Plant breeding
URN:NBN:urn:nbn:se:slu:epsilon-p-100496
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-100496
Additional ID:
Type of IDID
DOI10.1186/s13068-019-1479-7
ID Code:16206
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Department of Molecular Sciences
(S) > Department of Forest Biomaterials and Technology (from 131204)
(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:05 Jul 2019 06:15
Metadata Last Modified:05 Jul 2019 06:15

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