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Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses

Ekman, Stina and Björkman, Kristina and Löfgren, Maria and Skiöldebrand, Eva and Lindahl, A and Rüetschi, U. and Jansson, Anna and Abrahamsson-Aurell, K. and Björnsdottir, S. and Matsson Hulten, L. (2019). Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses. Equine Veterinary Journal. 51 , 674-680
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Abstract

Background: Molecular serum markers that can identify early reversible osteoarthritis (OA) in horses are lacking. Objectives: We studied serum concentrations of a novel cartilage oligomeric matrix protein (COMP) neo-epitope in horses subjected to short-term exercise and with acute lameness. The effects of circadian rhythm and age were also evaluated.
Study design: Longitudinal studies in healthy horses and cross-sectional comparison of lame and non-lame horses.
Methods: Sera were collected from five horses before and after short-term interval exercise and during full-day box rest. Sera from 32 acutely lame horses were used to evaluate age-related effects. Independent samples from control horses (n = 41) and horses with acute lameness (n = 71) were included. COMP neo-epitope concentrations were analysed using custom-developed inhibition ELISAs validated for equine serum. The presence of COMP neo-epitope was delineated in healthy and osteoarthritic articular cartilage with immunohistochemistry.
Results: COMP neo-epitope concentrations decreased after speed training but returned to baseline levels post-exercise. No correlations between age and serum COMP neo-epitope concentrations were found (r = 0.0013). The mean (s.d.) serum concentration of COMP neo-epitope in independent samples from non-lame horses was 0.84 0.38 lg/mL, and for lame horses was 5.24 1.83 lg/mL (P<0.001). Antibodies against COMP neo-epitope did not stain normal articular cartilage, but intracytoplasmic staining was found in superficial chondrocytes of mild OA cartilage and in the extracellular matrix of moderately osteoarthritic cartilage.
Main limitations: ELISA was based on polyclonal antisera rather than a monoclonal antibody. There is a sex and breed bias within the groups of horses, also it could have been of value to include horses with septic arthritis and tendonitis and investigated joint differences.
Conclusions: This COMP neo-epitope can be measured in sera, and results indicate that it could be a biomarker for pathologic fragmentation of cartilage in connection with acute joint lameness.

Authors/Creators:Ekman, Stina and Björkman, Kristina and Löfgren, Maria and Skiöldebrand, Eva and Lindahl, A and Rüetschi, U. and Jansson, Anna and Abrahamsson-Aurell, K. and Björnsdottir, S. and Matsson Hulten, L.
Title:Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses
Year of publishing :2019
Volume:51
Page range:674-680
Number of Pages:7
Publisher:John Wiley & Sons Ltd on behalf of EVJ Ltd
ISSN:0425-1644
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:horse, COMP neo-epitope, biomarker, lameness, serum, training, circadian
URN:NBN:urn:nbn:se:slu:epsilon-p-99534
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-99534
Additional ID:
Type of IDID
DOI10.1111/evj.13082
ID Code:16251
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:13 Aug 2019 07:10
Metadata Last Modified:13 Aug 2019 07:10

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