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Genetic and epigenetic mechanisms underlying the regulation of flowering time

Kralemann, Lejon (2019). Genetic and epigenetic mechanisms underlying the regulation of flowering time. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2019:54
ISBN 978-91-7760-426-6
eISBN 978-91-7760-427-3
[Doctoral thesis]

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Abstract

Developmental transitions and responses to the environment have a tight epigenetic control. Especially the switch to flowering is important for plants because it allows (sexual) reproduction, and should not occur unless the conditions are right. External and internal signals are relayed via FT/TFL1 genes; genes with a great impact on flowering time. The PRC1/PRC2 system plays an important role in the repression of flowering, indicated by the aberrant flowering phenotype of its mutants. Via deposition of histone modifications H2Aub1 and H3K27me3 it keeps genes stably silenced, so that transitions and responses do not happen as a result of random fluctuations in the internal or external environment.

In the first manuscript we focussed on PRC2-component MSI1 in Arabidopsis. MSI1 is the nucleosome-binding core component of PRC2 and other chromatin-modifying complexes. We found that MSI1 is also a core part of a histone de-acetylase complex together with histone de-acetylase HDA19. We further found that this complex represses the ABA-mediated salt stress response by de-acetylating the ABA receptors.

The second manuscript focused on flowering time in the invasive species Ambrosia artemisiifolia. During the last couple of centuries it invaded Europe, where it currently thrives mostly in the south-east. Earlier flowering populations have since been found in the north, suggesting local adaptation. We studied this early-flowering trait and found that it is inherited dominantly, and that it is maladaptive under long vegetation periods. We also identified the FT/TFL1 genes in this species, and found that a combination of expression changes in the FT-like floral activator and TFL1-like floral repressor likely underlies the altered flowering time.

In the third manuscript we tried to shed light on repressive H2A de-ubiquitination in Arabidopsis. Previously, it has been observed that loss of UBP12/13-mediated H2A deubiquitination causes loss of H3K27me3 and re-activation of some PRC2 targets. We show now that this holds true on a genome-wide level, and that the genes targeted by UBP12/13 are those affected in H3K27me3 maintenance and expression. We also showed that H2Aub1 not only recruits PRC2, but likely also recruits H3K27 demethylase REF6. We show that H2Aub1 therefore puts genes in a state responsive to stimuli, and that stable repression requires its removal.

Authors/Creators:Kralemann, Lejon
Title:Genetic and epigenetic mechanisms underlying the regulation of flowering time
Series/Journal:Acta Universitatis Agriculturae Sueciae (1652-6880)
Year of publishing :2019
Number:2019:54
Number of Pages:73
Papers/manuscripts:
NumberReferences
IMehdi, S; Derkacheva, M; Ramström, M; Kralemann, L; Bergquist, J; Hennig, L* (2016). The WD40 Domain Protein MSI1 Functions in a Histone Deacetylase Complex to Fine-Tune Abscisic Acid Signaling. The Plant Cell, vol 28, pp. 42–54.
IIKralemann, L⸸; Scalone, R⸸; Andersson, L; Hennig, L* (2018). North European invasion by common ragweed is associated with early flowering and dominant changes in FT/TFL1 expression. Journal of Experimental Botany, vol 69 (10), pp. 2647-2658.
IIIKralemann, L; Liu, S; Trejo-Arellano, M S; Muñoz Viana, R; Köhler, C*; Hennig, L. Removal of H2Aub1 by UBIQUITIN SPECIFIC PROTEASES 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis (submitted).
Place of Publication:Uppsala
Publisher:Department of Plant Biolgy, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-426-6
ISBN for electronic version:978-91-7760-427-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Full Text Status:Public
Agris subject categories.:F Plant production > F30 Plant genetics and breeding
F Plant production > F62 Plant physiology - Growth and development
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Arabidopsis, Ambrosia, FT, TFL1, epigenetics, histone modifications, PRC1, PRC2, UBP12, UBP13
URN:NBN:urn:nbn:se:slu:epsilon-p-101102
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-101102
ID Code:16266
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Deposited By: SLUpub Connector
Deposited On:14 Aug 2019 08:41
Metadata Last Modified:14 Aug 2019 08:41

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