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Virus dynamics in naturally Varroa-resistant honeybee populations

Thaduri, Srinivas (2019). Virus dynamics in naturally Varroa-resistant honeybee populations. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2019:62
ISBN 978-91-7760-442-6
eISBN 978-91-7760-443-3
[Doctoral thesis]

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Abstract

The ectoparasitic mite, Varroa destructor, together with its associated viruses is the most significant threat to honeybee (Apis mellifera) health world-wide. Since the introduction of varroa to the new host species, the European honey bee (Apis mellifera), it has been responsible for the near complete eradication of wild and feral honeybee populations in Europe and North America. However, a unique honeybee population on the island of Gotland, Sweden, has acquired resistance to the mite through a natural selection process. A recent study also showed that Gotland mite-resistant population might have adapted tolerance and resistance to virus infections. This suggests that virus-host interactions may play a key role in the long-term survival of this population. The aim of this thesis was to investigate the role of the viral and bacterial microbiome in the enhanced survival of the mite-resistant (MR) honeybees on Gotland, to compare the role of virus tolerance and resistance in other naturally selected mite-resistant honeybee populations, similar to the Gotland population, and to unravel individual level virus-host interactions in honeybees.

First, by using a combination of high-throughput sequencing and different bioinformatics tools we found Lake Sinai virus and Apis rhabdovirus-1, including previously known honey bee viruses, in Swedish honey bees. Further molecular studies showed that Gotland MR bees have developed a colony-level resistance to these viruses, and tolerance to Deformed Wing Virus (DWV), the virus most commonly associated with mite infestation. Secondly, differences in the bacterial microbiome between MR and mite-susceptible (MS) bees were studied using the 16S rDNA, but the results indicated little differences between MR and MS bees throughout the season. Finally, individual level susceptibility of MR and MS honey bees to oral virus infection was tested for DWV virus and Acute bee paralysis virus (ABPV). The results demonstrate that DWV and ABPV infection dynamics were nearly identical in MR and MS bees, but that bees from the MR honeybee populations had significantly lower mortality rates than bees from the MS population.

In conclusion, the results of this thesis present strong evidence that naturally adapted mite-resistant honeybees have also adapted, through a natural selection process, tolerance and resistance to virus infections at both the colony and individual level. The bacterial microbiome did not appear to play a role in the enhanced survival of Swedish mite-resistant honeybees but more studies are required to investigate potential bacteriavirus interactions on honeybee health. Future work should aim to identify key genomic regions associated with virus resistance and tolerance that can be incorporated into honeybee breeding programs to improve honeybee health.

Authors/Creators:Thaduri, Srinivas
Title:Virus dynamics in naturally Varroa-resistant honeybee populations
Series/Journal:Acta Universitatis Agriculturae Sueciae (1652-6880)
Year of publishing :2019
Number:2019:62
Number of Pages:73
Papers/manuscripts:
NumberReferences
IThaduri S.*, Locke B., Granberg F., de Miranda J. (2018). Temporal changes in the viromes of Swedish Varroa-resistant and Varroa-susceptible oneybee populations. PLoS ONE 13 (12): e0206938
IIThaduri S., Marupakula S., Terenius O., Locke B., de Miranda J.R.* (2019). Temporal dynamics in the bacterial and viral metagenomes of Swedish varroa-resistant and non-resistant honeybee populations. manuscript)
IIIThaduri S., Stephan J.G., de Miranda J.R., Locke B.* (2019). Disentangling host-parasite-pathogen interactions in a varroa-resistant honeybee population reveals virus tolerance as an independent, naturally adapted survival mechanism. Scientific Reports 9: 6221
IVLocke B.*, Thaduri S., Stephan J.G., Low M., Blacquière T., Dahle B., Le Conte Y., Neumann P., de Miranda J.R. (2019). Tolerance to experimental virus infections in four distinct varroa-resistant honeybee populations (manuscript)
Place of Publication:Uppsala
Publisher:Department of Ecology, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-442-6
ISBN for electronic version:978-91-7760-443-3
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Full Text Status:Public
Agris subject categories.:L Animal production > L20 Animal ecology
L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Zoology
Keywords:Apis mellifera, Varroa destructor, viral metagenomics, deformed wing virus, acute bee paralysis virus, virus-host interactions
URN:NBN:urn:nbn:se:slu:epsilon-p-101573
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-101573
ID Code:16331
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology
Deposited By: SLUpub Connector
Deposited On:17 Sep 2019 08:43
Metadata Last Modified:18 Sep 2019 14:12

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