Odilbekov, Firuz and He, Xinyao and Saripella, Ganapathi Varma and Armoniené, Rita and Henriksson, Tina and Singh, Pawan Kumar and Chawade, Aakash
(2019).
QTL Mapping and Transcriptome Analysis to Identify Differentially Expressed Genes Induced by Septoria Tritici Blotch Disease of Wheat.
Agronomy. 9
, 1-15
[Journal article]
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Abstract
Resistance to Septoria tritici blotch (STB) is an economically important trait in many wheat-breeding programs across the world. Several quantitative trait loci (QTL) for STB resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes/QTL and determine the underlying resistance mechanism. In this work, we integrated QTL mapping and transcriptome profiling to identify candidate genes underlying QTL associated with STB resistance in bread wheat at the seedling stage. The results revealed four QTL on chromosomes 1BS, 1BL, 3AS and 3DL for STB resistance. Among these, two QTL on 2BL and 3DL were mapped for chlorosis, necrosis and pycnidia while the other two on 1BS and 3AS were associated with necrosis and pycnidia. Among the four identified QTL, genes were identified in three QTL (1BS, 2BL and 3DL). In total, 238 differentially expressed genes (DEGs) were localized in 1BS, 16 DEGs in 2BL and 80 DEGs in 3DL QTL region respectively. F-box protein, NBS-LRR disease resistance genes and receptor-like protein kinase were the most over-represented. The results emphasize the importance of integrating QTL and transcriptome analysis to accelerate the identification of key genes underlying the traits of interest.
Authors/Creators: | Odilbekov, Firuz and He, Xinyao and Saripella, Ganapathi Varma and Armoniené, Rita and Henriksson, Tina and Singh, Pawan Kumar and Chawade, Aakash | ||||
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Title: | QTL Mapping and Transcriptome Analysis to Identify Differentially Expressed Genes Induced by Septoria Tritici Blotch Disease of Wheat | ||||
Year of publishing : | 2019 | ||||
Volume: | 9 | ||||
Page range: | 1-15 | ||||
Number of Pages: | 15 | ||||
Publisher: | MDPI | ||||
ISSN: | 2073-4395 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Refereed: | Yes | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||
Keywords: | septoria tritici blotch, Stb genes, wheat, transcriptomics, QTL analysis | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-101829 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-101829 | ||||
Additional ID: |
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ID Code: | 16365 | ||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||
Department: | (LTJ, LTV) > Department of Plant Breeding (from 130101) | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 11 Oct 2019 10:09 | ||||
Metadata Last Modified: | 28 Oct 2019 11:28 |
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