Abstract

Immunoassays are widely used for detection and quantification of analytes in biological samples, but are vulnerable to analytical errors caused by interfering sample substances. Of particular interest are endogenous anti-animal antibodies that may bind to the immunoassay antibodies and cause erroneous test results. This phenomenon is a hazard to patient safety in both human and veterinary medicine. Here, we demonstrate that anti-mouse antibodies in dogs bind selectively to different regions of the murine IgG molecule, cross-react with IgG from different species, and consist of all major antibody classes present in canine serum (IgA, IgG and IgM). The antibody characteristics varied among individuals and their prevalence differed between two dog breeds. The selective binding to different IgG regions suggests that the antibodies might not originate from immunization through exposure to mice or other species. These findings show that canine anti-mouse antibodies are highly heterogeneous in nature and therefore require a combination of strategies to be counteracted.

Keywords

immunoglobulins; immunoassay; immunization; biological samples; selective binding

Published in

Scientific Reports
2019, Volume: 9, article number: 14521

SLU Authors

• Bergman, Daniel

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Hamlin, Helene

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Ström Holst, Bodil

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science
Immunology


Publication identifier

DOI: https://doi.org/10.1038/s41598-019-51228-3

Permanent link to this page (URI)

https://res.slu.se/id/publ/102022