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Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference

Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Åhlén, Emma and Ström Holst, Bodil (2019). Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference. Scientific Reports. 9 , 1-9
[Journal article]

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Abstract

Immunoassays are widely used for detection and quantifcation of analytes in biological samples, but are vulnerable to analytical errors caused by interfering sample substances. Of particular interest are endogenous anti-animal antibodies that may bind to the immunoassay antibodies and cause erroneous test results. This phenomenon is a hazard to patient safety in both human and veterinary medicine. Here, we demonstrate that anti-mouse antibodies in dogs bind selectively to diferent regions of the murine IgG molecule, cross-react with IgG from diferent species, and consist of all major antibody classes present in canine serum (IgA, IgG and IgM). The antibody characteristics varied among individuals and their prevalence difered between two dog breeds. The selective binding to diferent IgG regions suggests that the antibodies might not originate from immunization through exposure to mice or other species. These fndings show that canine anti-mouse antibodies are highly heterogeneous in nature and therefore require a combination of strategies to be counteracted.

Authors/Creators:Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Åhlén, Emma and Ström Holst, Bodil
Title:Characterization of canine anti-mouse antibodies highlights that multiple strategies are needed to combat immunoassay interference
Year of publishing :2019
Volume:9
Page range:1-9
Number of Pages:9
Publisher:Nature Research
ISSN:2045-2322
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Immunology (Immunology in the medical area to be 30110)
Keywords:immunoglobulins, immunoassay, immunization, biological samples, selective binding
URN:NBN:urn:nbn:se:slu:epsilon-p-102022
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102022
Additional ID:
Type of IDID
DOI10.1038/s41598-019-51228-3
ID Code:16383
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:14 Nov 2019 09:51
Metadata Last Modified:14 Nov 2019 09:51

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