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Investigation of interference from canine anti-mouse antibodies in hormone immunoassays

Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Ström Holst, Bodil (2019). Investigation of interference from canine anti-mouse antibodies in hormone immunoassays. Veterinary Clinical Pathology. 48 , 59-69
[Journal article]

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Abstract

Background: Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Mullerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.
Objectives: We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.
Methods: Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT4), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.
Results: Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%). Conclusions Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.

Authors/Creators:Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Ström Holst, Bodil
Title:Investigation of interference from canine anti-mouse antibodies in hormone immunoassays
Year of publishing :2019
Volume:48
Page range:59-69
Number of Pages:11
Publisher:Wiley
ISSN:0275-6382
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Clinical Laboratory Medicine
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Endocrinology and Diabetes
Keywords:antibody, anti-Mullerian hormone, canine, PEG, polyethylene glycol
URN:NBN:urn:nbn:se:slu:epsilon-p-100741
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-100741
Additional ID:
Type of IDID
DOI10.1111/vcp.12764
Web of Science (WoS)000476103200001
ID Code:16384
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:29 Oct 2019 10:02
Metadata Last Modified:29 Oct 2019 10:02

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