Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Ström Holst, Bodil
(2019).
Investigation of interference from canine anti-mouse antibodies in hormone immunoassays.
Veterinary Clinical Pathology. 48
, 59-69
[Research article]
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Abstract
Background: Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Mullerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.
Objectives: We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.
Methods: Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT4), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.
Results: Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%). Conclusions Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.
| Authors/Creators: | Bergman, Daniel and Larsson, Anders and Hansson-Hamlin, Helene and Ström Holst, Bodil | ||||||
|---|---|---|---|---|---|---|---|
| Title: | Investigation of interference from canine anti-mouse antibodies in hormone immunoassays | ||||||
| Series Name/Journal: | Veterinary Clinical Pathology | ||||||
| Year of publishing : | 2019 | ||||||
| Volume: | 48 | ||||||
| Page range: | 59-69 | ||||||
| Number of Pages: | 11 | ||||||
| Publisher: | Wiley | ||||||
| ISSN: | 0275-6382 | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Refereed: | Yes | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution-Noncommercial 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Clinical Laboratory Medicine (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 302 Clinical Medicine > Endocrinology and Diabetes | ||||||
| Keywords: | antibody, anti-Mullerian hormone, canine, PEG, polyethylene glycol | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-100741 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-100741 | ||||||
| Additional ID: |
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| ID Code: | 16384 | ||||||
| Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
| Department: | (VH) > Dept. of Clinical Sciences | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 29 Oct 2019 10:02 | ||||||
| Metadata Last Modified: | 29 Oct 2019 10:02 |
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