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Filamentous fungi in wrapped forages

Schenck, Jessica (2019). Filamentous fungi in wrapped forages. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2019:69
ISBN 978-91-7760-456-3
eISBN 978-91-7760-457-0
[Doctoral thesis]

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Abstract

Wrapped forages of higher dry matter (DM) concentrations (> 50 %), also referred to as haylage are common in Sweden and Norway. Such forages are preserved by a combination of semi-drying and anaerobic storage leading to an environment that may affect the composition of filamentous fungi differently than in hay or silage. The aim of this research was to identify fungal species in wrapped forages with higher DM concentration in relation to forage production and management factors.

In the first study, the effect of plant maturity at harvest on microbial composition of forage was investigated. The microbial composition of fresh herbage and conserved haylage was compared for three different harvest times (June, July and August) of the first cut of the season. The fungal load increased with later harvest dates in haylage, but fungal species detected in the herbage were not detected in the haylage. In the second study, bales from 124 farms were sampled, and data on production factors, chemical composition and mycotoxin presence included. Samples for analysis of fungi were taken from patches with visible fungal growth on the bale surfaces, and from drilled samples from the forage. Results showed a higher risk of fungal presence with increasing DM concentration, or if less than eight layers of polyethylene stretch film were used for wrapping. Presence of mycotoxins and their respective fungal species were not correlated (P>0.05). However, higher fungal counts were positively correlated with presence of mycotoxins.

Ocular inspection and cultivation for identification of fungal species is timeand labour consuming and has inherent difficulties. Therefore, identification of fungal species by extracting fungal DNA directly from forage samples is of interest. A study on three new primers in the fungal ITS (internal transcribed spacer) region for 454-sequencing was performed. Results showed that not all fungal species can be identified in the ITS-region and therefore other DNA regions are of interest.

Authors/Creators:Schenck, Jessica
Title:Filamentous fungi in wrapped forages
Series/Journal:Acta Universitatis Agriculturae Sueciae (1652-6880)
Year of publishing :2019
Number:2019:69
Number of Pages:75
Papers/manuscripts:
NumberReferences
ISchenck J. and *Müller C.E. 2014. Microbial composition pre- and postconservation of grass-dominated haylage harvested early, middle and late in the season. Journal of Equine Veterinary Sciences 34, 593-601.
IISchenck J., Djurle A., Jensen Funck D., Müller C., O’Brien M. and *Spörndly R. 2019. Filamentous fungi in wrapped forages determined with different sampling and culturing methods. Grass and Forage Science 74, 29-41.
IIISchenck J, Müller C., Djurle A., Funck Jensen D., O’Brien M., Johansen A., Rasmussen P H. and *Spörndly R. 2019. Occurrence of filamentous fungi and mycotoxins in wrapped forages in Sweden and Norway and their relation to chemical composition and management (accepted in Grass and Forage Science).
IVIhrmark K., Bödeker I.T., Cruz-Martinez K., Friberg H., Kubartova A., Schenck J., Strid Y., Stenlid J., Brandström-Durling M., Clemmensen K.E. and *Lindahl B.D. 2012. New primers to amplify the fungal ITS2 region – evaluation by 454-sequencing of artificial and natural communities. FEMS Microbiol Ecol 82, 666-677.
Place of Publication:Uppsala
Publisher:Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-456-3
ISBN for electronic version:978-91-7760-457-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Full Text Status:Public
Agris subject categories.:H Protection of plants and stored products > H20 Plant diseases
L Animal production > L02 Animal feeding
Q Food science > Q52 Feed processing and preservation
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:filamentous fungi, mould, wrapped forages, haylage and mycotoxins
URN:NBN:urn:nbn:se:slu:epsilon-p-102345
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102345
ID Code:16413
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Institutionen för husdjurens utfodring och vård
Deposited By: SLUpub Connector
Deposited On:29 Oct 2019 14:13
Metadata Last Modified:31 Oct 2019 09:20

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