Ustariz Olivera, Karina Rossio
(2019).
Building knowledge for a forage breeding program on native Festuca species in the highlands of Bolivia.
Diss. (sammanfattning/summary)
Alnarp :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
ISBN 978-91-7760-470-9
eISBN 978-91-7760-471-6
[Doctoral thesis]
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Abstract
Native grasses constitute the most important source of feed for camelids, sheep and cattle in the highlands of Bolivia, where the genus Festuca is one of the major feed components. The two most important species of this region are F. dolichophylla J. Presl (known as “chilliwa”) and F. orthophylla Pilg (known as “iru ichu”). This research aimed to investigate the phylogenetic relationships within the Festuca genus from the highlands of Bolivia, to evaluate the genetic diversity using EST-SSR markers and to assess the nutritional value for its use in breeding and conservation. The phylogenetic analysis, which was based on sequences derived from nuclear (ITS, CEN, Acc1) and chloroplast DNA (matK) regions, revealed that the Bolivian Festuca species derived from a common ancestor of the fine-leaved lineage of the Festuca sub-genus. In addition, Bolivian fescues where all grouped together, thus indicating that they belong to the American clade II of the Loliinae subtribe. The evaluation of genetic diversity performed using 12 EST-SSR markers in 43 populations indicated absence of clear population structure. However, four primer-pairs (FES 04, FES 13, FES 24 and NFA 142) were at the top in their polymorphism and gene diversity indicating the putative use of them in future population genetic analysis. Moreover, they could be helpful in pursuing the development of effective conservation management strategies for native and endangered Festuca species in Bolivia. The assessment of the mineral composition, protein, ash and cellulose contents, which were determined in 11 Bolivian Festuca ecotypes and 2 cultivars from Argentina, revealed as candidates for genetic improvement, the accessions 29, 10, 21, 32, 38 and 23 due to their high protein, Ca, Mg and P contents. The result generated in this work represents the starting material to develop a forage breeding program of the Festuca species in Bolivia as well as develop strategies for ex situ and in situ germplasm conservation.
| Authors/Creators: | Ustariz Olivera, Karina Rossio | ||||||||
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| Title: | Building knowledge for a forage breeding program on native Festuca species in the highlands of Bolivia | ||||||||
| Alternative abstract: |
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| Series Name/Journal: | Acta Universitatis Agriculturae Sueciae | ||||||||
| Year of publishing : | 2019 | ||||||||
| Number: | 2019:76 | ||||||||
| Number of Pages: | 58 | ||||||||
| Papers/manuscripts: |
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| Place of Publication: | Alnarp | ||||||||
| Publisher: | Department of Plant Breeding, Swedish University of Agricultural Sciences | ||||||||
| ISBN for printed version: | 978-91-7760-470-9 | ||||||||
| ISBN for electronic version: | 978-91-7760-471-6 | ||||||||
| ISSN: | 1652-6880 | ||||||||
| Language: | English | ||||||||
| Publication Type: | Doctoral thesis | ||||||||
| Article category: | Other scientific | ||||||||
| Full Text Status: | Public | ||||||||
| Agris subject categories.: | F Plant production > F30 Plant genetics and breeding | ||||||||
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding | ||||||||
| Keywords: | EST-SSR, Festuca, Genetic diversity, Nutritional value, Phylogeny | ||||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-102453 | ||||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102453 | ||||||||
| ID Code: | 16423 | ||||||||
| Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||||||
| Department: | (LTJ, LTV) > Department of Plant Breeding (from 130101) | ||||||||
| Deposited By: | SLUpub Connector | ||||||||
| Deposited On: | 05 Nov 2019 10:05 | ||||||||
| Metadata Last Modified: | 05 Nov 2019 10:05 |
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