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Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes

Desta, Zeratsion Abera and Kolano, Bozena and Shamim, Zeeshan and Armstrong, Susan J. and Rewers, Monika and Sliwinska, Elwira and Kushwaha, Sandeep Kumar and Parkin, Isobel A. P. and Ortiz Rios, Rodomiro Octavio and De Koning, Dirk-Jan (2019). Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes. Scientific Reports. 9 , 1-14
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Abstract

Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant, has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting annotated genome assemblies and positional cloning of candidate genes. Our principal goal was to construct a genetic map using integrated approaches of genetic, comparative and cytogenetic map analyses. In total, 503 F2 interspecifc hybrid individuals were genotyped using 7,624 single nucleotide polymorphism markers. Comparative analysis demonstrated that ~57% of the sequenced loci in L. campestre were congruent with Arabidopsis thaliana (L.) genome and suggested a novel karyotype, which predates the ancestral crucifer karyotype. Aceto-orcein chromosome staining and fuorescence in situ hybridization (FISH) analyses confrmed that L. campestre, L. heterophyllum Benth. and their hybrids had a chromosome number of 2n=2x=16. Flow cytometric analysis revealed that both species possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative maps with cytogenetic map analyses assigned two linkage groups to their particular chromosomes. Future work could incorporate FISH utilizing A. thaliana mapped BAC clones to allow the chromosomes of feld cress to be identifed reliably.

Authors/Creators:Desta, Zeratsion Abera and Kolano, Bozena and Shamim, Zeeshan and Armstrong, Susan J. and Rewers, Monika and Sliwinska, Elwira and Kushwaha, Sandeep Kumar and Parkin, Isobel A. P. and Ortiz Rios, Rodomiro Octavio and De Koning, Dirk-Jan
Title:Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes
Year of publishing :2019
Volume:9
Page range:1-14
Number of Pages:14
Publisher:Nature research
ISSN:2045-2322
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Bioinformatics and Systems Biology (methods development to be 10203)
Keywords:Lepidium campestre L., Field cress, genome mapping, oilseed plants
URN:NBN:urn:nbn:se:slu:epsilon-p-102655
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102655
Additional ID:
Type of IDID
DOI10.1038/s41598-019-53320-0
ID Code:16453
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
VH - Faculty of Veterinary Medicine and Animal Science
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:25 Nov 2019 07:12
Metadata Last Modified:21 Aug 2020 00:09

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