Home About Browse Search
Svenska


Giardia excretory-secretory proteins modulate the enzymatic activities of mast cell chymase and tryptase

Li, Zhiqiang and Peirasmaki, Dimitra and Svard, Staffan and Åbrink, Magnus (2019). Giardia excretory-secretory proteins modulate the enzymatic activities of mast cell chymase and tryptase. Molecular Immunology. 114 , 535-544
[Journal article]

[img] PDF - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

1MB

Abstract

Background: Mast cells are involved in the host immune response controlling infection with the non-invasive intestinal protozoan parasite Giardia intestinalis. Experimental infections in rodents with G. intestinalis showed increased intestinal expression of mucosal and connective mast cell specific proteases suggesting that both mucosal and connective tissue mast cells are recruited and activated during infection. During infection Giardia excretory-secretory proteins (ESPs) with immunomodulatory capacity are released. However, studies investigating potential interactions between Giardia ESPs and the connective tissue mast cell specific serine proteases, ie. human chymase and mouse mast cell protease (mMCP)-4 and, human and mouse tryptase (mMCP-6) remain scarce.Results: We first investigated if soluble Giardia proteins (sGPs), which over-lap extensively in protein content with ESP fractions, from the isolates GS, WB and H3, could induce mast cell activation. sGPs induced a minor activation of bone marrow derived mucosal-like mast cells, as indicated by increased IL-6 secretion and no degranulation. Furthermore, sGPs were highly resistant to degradation by human tryptase while human chymase degraded a 65 kDa sGP and, wild-type mouse ear tissue extracts degraded several protein bands in the 10 to 75 kDa range. In striking contrast, sGPs and ESPs were found to increase the enzymatic activity of human and mouse tryptase and to reduce the activity of human and mouse chymase.Conclusion: Our finding suggests that Giardia ssp. via enhancement or reduction of mast cell protease activity may modulate mast cell-driven intestinal immune responses. ESP-mediated modulation of the mast cell specific proteases may also increase degradation of tight junctions, which may be beneficial for Giardia ssp. during infection.

Authors/Creators:Li, Zhiqiang and Peirasmaki, Dimitra and Svard, Staffan and Åbrink, Magnus
Title:Giardia excretory-secretory proteins modulate the enzymatic activities of mast cell chymase and tryptase
Year of publishing :2019
Volume:114
Page range:535-544
Number of Pages:10
Publisher:Elsevier
ISSN:0161-5890
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Accepted version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Cell and Molecular Biology
Keywords:Mast cell, Tryptase, Chymase, Infection, Giardia ssp., Parasite, Intestine, Giardia excretory-secretory proteins (ESPs)
URN:NBN:urn:nbn:se:slu:epsilon-p-102397
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102397
Additional ID:
Type of IDID
DOI10.1016/j.molimm.2019.07.024
Web of Science (WoS)000490625600050
ID Code:16459
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:27 Jan 2020 12:29
Metadata Last Modified:10 Sep 2020 23:15

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits