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Mastitis in dairy cows in Rwanda

prevalence, aetiology, antimicrobial resistance, molecular epidemiology and effects on milk quality

Ndahetuye, Jean-Baptiste (2019). Mastitis in dairy cows in Rwanda. Diss. (sammanfattning/summary) Uppsala : Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2019:77
ISBN 978-91-7760-472-3
eISBN 978-91-7760-473-0
[Doctoral thesis]

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Abstract

The milk sector in Rwanda can be made competitive through improved udder health resulting in higher milk yields. This thesis investigated prevalence and aetiology of subclinical mastitis (SCM) in dairy cows, antimicrobial resistance and molecular epidemiology of udder pathogens. Screening for SCM with California Mastitis Test (CMT) was done in 828 cows in 429 herds from five regions in Rwanda. Milk was sampled from udder quarters with CMT score ≥3. Herd bulk milk quality and safety was investigated to generate knowledge for quality control. Overall SCM prevalence was 70.4% on herd level, 66.3% on cow level and 39% on quarter level. Overall 73.9% of all cultured milk samples were bacteriologically positive. Non-aureus staphylococci (NAS) followed by Staphylococcus (S.) aureus were the predominant pathogens. Staphylococcus chromogenes, epidermidis and sciuri were the most prevalent NAS. There was a high diversity of S. aureus sequence types, with both humans and cows as possible sources. Penicillin resistance exceeded 60% in all staphylococci. Among S. aureus isolates, 83.3% were resistant to penicillin, 100% to clindamycin and 20% to tetracycline. Main risk factors for SCM with implications on management routines included housing of cows in individual cattle kraal and on earthen floor, poor hygiene (hands, cows and milking area), absence of foremilk stripping, increasing stage of lactation, Holstein breed, lack of calf suckling and of feeding after milking. Total bacterial count and somatic cell count was high in milk from farms and milk collection centers, which indicate poor udder health and hygiene and contamination along the transport chain. Presence of Escherichia coli, Salmonella spp. and brucella antibodies in milk was common. Antimicrobial residues in milk was uncommon. In conclusion, SCM is common in dairy herds in Rwanda and the majority of causative pathogens exhibited penicillin resistance. The high microbial load has implications for milk quality, processability and public health. The high genetic diversity of S. aureus should be considered in future studies of disease spread. A mastitis control plan is recommended.

Authors/Creators:Ndahetuye, Jean-Baptiste
Title:Mastitis in dairy cows in Rwanda
Subtitle:prevalence, aetiology, antimicrobial resistance, molecular epidemiology and effects on milk quality
Series/Journal:Acta Universitatis Agriculturae Sueciae (1652-6880)
Year of publishing :2019
Number:2019:77
Number of Pages:89
Papers/manuscripts:
NumberReferences
INdahetuye*, J. B., Persson, Y., Nyman, A.-K., Tukei, M., Ongol, M. P., & Båge, R (2019). Aetiology and prevalence of subclinical mastitis in dairy herds in peri-urban areas of Kigali in Rwanda. Tropical Animal Health and Production, 51, 2019, pp. 2037–2044.
IINdahetuye*, J. B., Twambazimana, J., Karege, C., Persson, Y., Nyman, A.-K., Tukei, M., Ongol, M. P., Båge, R. A cross sectional study of prevalence and risk factors associated with subclinical mastitis and intramammary infections, in dairy herds linked to milk collection centers in Rwanda (accepted for publication in Preventive Veterinary Medicine)
IIINdahetuye, J.B., Ingabire, A., Nyman, A.-K., Karege C., Artursson K., Ongol, M.,P.,Tukei, M., Båge, R., Persson, Y. Microbiological quality and safety of milk from farm to milk collection centers in Rwanda. (manuscript)
IVNdahetuye, J.B., Leijon, M., Artursson K., Båge, R., Persson, Y. Genetic characterization of Staphylococcus aureus from subclinical mastitis cases in dairy cows in Rwanda (manuscript)
Place of Publication:Uppsala
Publisher:Department of Clinical Sciences, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-472-3
ISBN for electronic version:978-91-7760-473-0
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Full Text Status:Public
Agris subject categories.:L Animal production > L73 Animal diseases
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Genetics and Breeding
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:antimicrobial susceptibility, MALDI-TOF, intramammary infection, whole genome sequencing
URN:NBN:urn:nbn:se:slu:epsilon-p-102677
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102677
ID Code:16465
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:27 Nov 2019 12:44
Metadata Last Modified:13 Jan 2020 11:54

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