Johansen, Ida Elisabeth and Liu, Ying and Jørgensen, Bodil and Bennett, Eric Paul and Andreasson, Erik and Nielsen, Kåre L. and Blennow, Andreas and Larsen Petersen, Bent
(2019).
High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato.
Scientific Reports. 9
, 1-7
[Journal article]
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Official URL: https://www.nature.com/articles/s41598-019-54126-w
Abstract
CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.
| Authors/Creators: | Johansen, Ida Elisabeth and Liu, Ying and Jørgensen, Bodil and Bennett, Eric Paul and Andreasson, Erik and Nielsen, Kåre L. and Blennow, Andreas and Larsen Petersen, Bent | ||||
|---|---|---|---|---|---|
| Title: | High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato | ||||
| Year of publishing : | 2019 | ||||
| Volume: | 9 | ||||
| Page range: | 1-7 | ||||
| Number of Pages: | 7 | ||||
| Publisher: | NATURE PUBLISHING GROUP | ||||
| ISSN: | 2045-2322 | ||||
| Language: | English | ||||
| Publication Type: | Journal article | ||||
| Refereed: | Yes | ||||
| Article category: | Scientific peer reviewed | ||||
| Version: | Published version | ||||
| Copyright: | Creative Commons: Attribution 4.0 | ||||
| Full Text Status: | Public | ||||
| Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology | ||||
| Keywords: | Nuclear RNA gene, guide RNA, genome, expression, sequence, Arabidopsis, prediction, resistance, mutation, cloning | ||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-102773 | ||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102773 | ||||
| Additional ID: |
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| ID Code: | 16468 | ||||
| Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||
| Department: | (LTJ, LTV) > Department of Plant Protection Biology | ||||
| Deposited By: | SLUpub Connector | ||||
| Deposited On: | 19 Dec 2019 13:14 | ||||
| Metadata Last Modified: | 19 Dec 2019 13:14 |
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