Home About Browse Search
Svenska


High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

Johansen, Ida Elisabeth and Liu, Ying and Jørgensen, Bodil and Bennett, Eric Paul and Andreasson, Erik and Nielsen, Kåre L. and Blennow, Andreas and Larsen Petersen, Bent (2019). High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato. Scientific Reports. 9 , 1-7
[Journal article]

[img] PDF - Published Version
Available under License Creative Commons Attribution.

1MB

Official URL: https://www.nature.com/articles/s41598-019-54126-w

Abstract

CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular Bound Starch Synthase gene at the protoplast level were obtained by replacement of the Arabidopsis U6 promotor, driving expression of the CRISPR component, with endogenous potato U6 promotors. This translated at the ex-plant level into 35% full allelic gene editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment of gene editing in complex genomes, such as potato. Together, this warrants significant reduction of laborious cell culturing, ex-plant regeneration and screening procedures of plants with high complexity genomes.

Authors/Creators:Johansen, Ida Elisabeth and Liu, Ying and Jørgensen, Bodil and Bennett, Eric Paul and Andreasson, Erik and Nielsen, Kåre L. and Blennow, Andreas and Larsen Petersen, Bent
Title:High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato
Year of publishing :2019
Volume:9
Page range:1-7
Number of Pages:7
Publisher:NATURE PUBLISHING GROUP
ISSN:2045-2322
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
Keywords:Nuclear RNA gene, guide RNA, genome, expression, sequence, Arabidopsis, prediction, resistance, mutation, cloning
URN:NBN:urn:nbn:se:slu:epsilon-p-102773
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-102773
Additional ID:
Type of IDID
DOI10.1038/s41598-019-54126
ID Code:16468
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:19 Dec 2019 13:14
Metadata Last Modified:19 Dec 2019 13:14

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits