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In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins

Ramin, Mohammad and De Oliveira Franco, Marcia and Roleda, Michael Y. and Aasen, Inga Marie and Hetta, Mårten and Steinshamn, Håvard (2019). In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins. Animal Feed Science and Technology. 255 , 1-12
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Abstract

Utilisable crude protein (uCP), methane (CH4) production and other fermentation parameters were analysed in vitro for a diet in which grass silage was replaced by different levels of seaweed protein fractions prepared from three seaweed species: Saccharina latissima, Alaria esculenta and Palmaria palmata. Ten fractions from these three species in which the protein content had been increased and the salt content reduced by simple processing were tested, with inclusion levels in the diet based on the nitrogen content of the fractions. Following an extraction procedure, four fractions from Saccharina latissima, three from Alaria esculenta and one from Palmaria palmata, were incrementally included in the diet by replacing high quality silage with approximately 0, 0.15, 0.30 and 0.45 g/g DM, while two high-protein fractions of Palmaria palmata were tested at replacement levels of 0, 0.075, 0.15 and 0.225 g/g DM. To estimate fermentation parameters, 500 mg of each diet were incubated in bottles with 60 mL buffered rumen fluid. Estimated uCP increased linearly with increasing replacement rate of grass silage with seaweed protein fractions (from 158 g/kg DM to 206 g/kg DM on average for all fractions). Increasing protein fraction from the brown seaweed Saccharina latissima in the diet significantly increased true organic matter digestibility (OMD) (from on average 0.786 to 0.821). Organic matter digestibility decreased with increasing level of Alaria esculenta fractions (from on average 0.785 to 0.733), which also gave a linear decrease in CH4 production (from on average 45.3 to 38.5 mL/g organic matter). As a result of decreased CH4 production and OMD, total volatile fatty acid concentration decreased with increasing level of Alaria esculenta fractions (from on average 69.5 to 63.0 mmol/L). Thus, positive and species-specific effects of seaweed on estimated uCP and fermentation parameters were observed in vitro when protein fractions remaining after an extraction procedure on seaweed partly replaced grass silage in the feed ration.

Authors/Creators:Ramin, Mohammad and De Oliveira Franco, Marcia and Roleda, Michael Y. and Aasen, Inga Marie and Hetta, Mårten and Steinshamn, Håvard
Title:In vitro evaluation of utilisable crude protein and methane production for a diet in which grass silage was replaced by different levels and fractions of extracted seaweed proteins
Year of publishing :2019
Volume:255
Page range:1-12
Number of Pages:12
Publisher:Elsevier
ISSN:0377-8401
Language:English
Publication Type:Journal article
Refereed:Yes
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 402 Animal and Dairy Science > Animal and Dairy Science.
Keywords:Extracted protein, Fermentation parameters, Organic matter digestibility
URN:NBN:urn:nbn:se:slu:epsilon-p-103014
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-103014
Additional ID:
Type of IDID
DOI10.1016/j.anifeedsci.2019.114225
ID Code:16489
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Agricultural Research for Northern Sweden
(VH) > Dept. of Agricultural Research for Northern Sweden
Deposited By: SLUpub Connector
Deposited On:03 Feb 2020 12:29
Metadata Last Modified:03 Feb 2020 12:29

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