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New molecular methods to assess biodiversity. Potentials and pitfalls of DNA metabarcoding: a workshop report

Kahlert, Maria and Alfjorden, Anders and Apunte-Ramos, Katherine and Bailet, Bonnie and Burillo, Javier Pérez and Gonzalez, Andrea Greta Carrera and Castro, David and Di Bernardi, Cecilia and Dully, Verena and Fekete, Judit and Frühe, Larissa and González, Raquel and Gratsia, Eirini and Hanjalic, Jasna and Kamberovic, Jasmina and Kelly, Ann-Marie and Meriggi, Carlotta and Nousianen, Ilkka and Øberg, Sarah B. and Orr, James and Quintana, Cintia and Papatheodoulou, Athina and Sargac, Jasmina and Shahbaz, Muhammad and Tapolczai, Kalman and Tosic, Katarina and Wallin, Isa and Zupancic, Masa and Bohman, Patrik and Buttigieg, Pier Luigi and Häubner, Norbert and Leese, Florian and Macher, Jan and Peura, Sari and Roslin, Tomas and Strand, Malin and Terenius, Olle and Vasselon, Valentiin and Weigand, Alexander M. (2019). New molecular methods to assess biodiversity. Potentials and pitfalls of DNA metabarcoding: a workshop report. Research Ideas and Outcomes. 5 , 1-9
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Abstract

This report presents the outcome of the joint work of PhD students and senior researchers working with DNA-based biodiversity assessment approaches with the goal to facilitate others the access to definitions and explanations about novel DNA-based methods. The work was performed during a PhD course (SLU PNS0169) at the Swedish University of Agricultural Sciences (SLU) in Uppsala, Sweden. The course was co-organized by the EU COST research network DNAqua-Net and the SLU Research Schools Focus on Soils and Water (FoSW) and Ecology - basics and applications. DNAqua-Net (COST Action CA15219, 2016-2020) is a network connecting researchers, water managers, politicians and other stakeholders with the aim to develop new genetic tools for bioassessment of aquatic ecosystems in Europe and beyond. The PhD course offered a comprehensive overview of the paradigm shift from traditional morphology-based species identification to novel identification approaches based on molecular markers. We covered the use of molecular tools in both basic research and applied use with a focus on aquatic ecosystem assessment, from species collection to the use of diversity in environmental legislation. The focus of the course was on DNA (meta)barcoding and aquatic organisms. The knowledge gained was shared with the general public by creating Wikipedia pages and through this collaborative Open Access publication, co-authored by all course participants.

Authors/Creators:Kahlert, Maria and Alfjorden, Anders and Apunte-Ramos, Katherine and Bailet, Bonnie and Burillo, Javier Pérez and Gonzalez, Andrea Greta Carrera and Castro, David and Di Bernardi, Cecilia and Dully, Verena and Fekete, Judit and Frühe, Larissa and González, Raquel and Gratsia, Eirini and Hanjalic, Jasna and Kamberovic, Jasmina and Kelly, Ann-Marie and Meriggi, Carlotta and Nousianen, Ilkka and Øberg, Sarah B. and Orr, James and Quintana, Cintia and Papatheodoulou, Athina and Sargac, Jasmina and Shahbaz, Muhammad and Tapolczai, Kalman and Tosic, Katarina and Wallin, Isa and Zupancic, Masa and Bohman, Patrik and Buttigieg, Pier Luigi and Häubner, Norbert and Leese, Florian and Macher, Jan and Peura, Sari and Roslin, Tomas and Strand, Malin and Terenius, Olle and Vasselon, Valentiin and Weigand, Alexander M.
Title:New molecular methods to assess biodiversity. Potentials and pitfalls of DNA metabarcoding: a workshop report
Year of publishing :2019
Volume:5
Page range:1-9
Number of Pages:9
Publisher:Pensoft
ISSN:2367-7163
Language:English
Publication Type:Other publication in scientific journal
Refereed:No
Article category:Other scientific
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Ecology
Keywords:DNA barcoding, biodiversity, molecular identification, aquatic organisms, metabarcoding
URN:NBN:urn:nbn:se:slu:epsilon-p-101553
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-101553
Additional ID:
Type of IDID
DOI10.3897/rio.5.e38915
ID Code:16676
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Aquatic Sciences and Assessment
(NL, NJ) > Dept. of Soil and Environment
(S) > Dept. of Soil and Environment

(NL, NJ) > Dept. of Ecology
(S) > Dept. of Ecology

(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:05 Mar 2020 13:21
Metadata Last Modified:05 Mar 2020 13:21

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