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Nitrogen source-dependent inhibition of yeast growth by glycine and its N-methylated derivatives

Linder, Tomas (2020). Nitrogen source-dependent inhibition of yeast growth by glycine and its N-methylated derivatives. Antonie van Leeuwenhoek. 113 , 437-445
[Journal article]

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Abstract

The effect of nitrogen source on the inhibitory properties of glycine and its N-methylated derivatives N-methylglycine (sarcosine), N,N-dimethylglycine, N,N,N-trimethylglycine (glycine betaine) on yeast growth was investigated. On solid minimal medium, all four glycine species completely or partially inhibited growth of Kluyveromyces lactis, Komagataella pastoris, Ogataea arabinofermentans, Spathaspora passalidarum and Yamadazyma tenuis at concentrations 5-10 mM when 10 mM NH4Cl was the sole source of nitrogen. If NH4Cl was substituted by sodium L-glutamate as the sole source of nitrogen, obvious growth inhibition by glycine and its N-methylated derivatives was generally not observed in any of these species. No obvious growth inhibition by any of the glycine species at a concentration of 10 mM was observed in Cyberlindnera jadinii, Lipomyces starkeyi, Lodderomyces elongisporus, Scheffersomyces stipitis or Yarrowia lipolytica on solid minimal medium irrespective of whether the nitrogen source was NH4Cl or sodium L-glutamate. Growth inhibition assays of K. pastoris in liquid minimal medium supplemented with increasing concentrations of N,N-dimethylglycine demonstrated inhibitory effects for nine tested nitrogen sources. In most cases, N,N-dimethylglycine supplementation caused a decrease in growth efficiency that appeared to be proportional to the concentration of N,N-dimethylglycine. The biological relevance of these results is discussed.

Authors/Creators:Linder, Tomas
Title:Nitrogen source-dependent inhibition of yeast growth by glycine and its N-methylated derivatives
Year of publishing :2020
Volume:113
Page range:437-445
Number of Pages:9
Publisher:Springer
ISSN:0003-6072
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Microbiology (Microbiology in the medical area to be 30109)
Keywords:Adaptive response, Antifungal, Metabolism, Phenotype, Yeast
URN:NBN:urn:nbn:se:slu:epsilon-p-103200
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-103200
Additional ID:
Type of IDID
DOI10.1007/s10482-019-01342-z
Web of Science (WoS)000491524500002
ID Code:16708
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Department of Molecular Sciences
Deposited By: SLUpub Connector
Deposited On:26 Mar 2020 14:16
Metadata Last Modified:26 Mar 2020 14:16

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