Fredriksson, Robert and Sreedharan, Smitha and Nordenankar, Karin and Alsio, Johan and Lindberg, Frida A. and Hutchinson, Ashley and Eriksson, Anders and Roshanbin, Sahar and Ciuculete, Diana M. and Klockars, Anica and Todkar, Aniruddha and Hagglund, Maria G. and Hellsten, Sofie and Hindlycke, Viktoria and Vastermark, Ake and Shevchenko, Ganna and Olivo, Gaia and Cheng, Ken and Kullander, Klas and Moazzami, Ali and Bergquist, Jonas and Olszewski, Pawel K. and Schioth, Helgi B.
(2019).
The polyamine transporter Slc18b1(VPAT) is important for both short and long time memory and for regulation of polyamine content in the brain.
PLoS Genetics. 15
, 1-30
[Research article]
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Abstract
SLC18B1 is a sister gene to the vesicular monoamine and acetylcholine transporters, and the only known polyamine transporter, with unknown physiological role. We reveal that Slc18b1 knock out mice has significantly reduced polyamine content in the brain providing the first evidence that Slc18b1 is functionally required for regulating polyamine levels. We found that this mouse has impaired short and long term memory in novel object recognition, radial arm maze and self-administration paradigms. We also show that Slc18b1 KO mice have altered expression of genes involved in Long Term Potentiation, plasticity, calcium signalling and synaptic functions and that expression of components of GABA and glutamate signalling are changed. We further observe a partial resistance to diazepam, manifested as significantly lowered reduction in locomotion after diazepam treatment. We suggest that removal of Slc18b1 leads to reduction of polyamine contents in neurons, resulting in reduced GABA signalling due to long-term reduction in glutamatergic signalling.
| Authors/Creators: | Fredriksson, Robert and Sreedharan, Smitha and Nordenankar, Karin and Alsio, Johan and Lindberg, Frida A. and Hutchinson, Ashley and Eriksson, Anders and Roshanbin, Sahar and Ciuculete, Diana M. and Klockars, Anica and Todkar, Aniruddha and Hagglund, Maria G. and Hellsten, Sofie and Hindlycke, Viktoria and Vastermark, Ake and Shevchenko, Ganna and Olivo, Gaia and Cheng, Ken and Kullander, Klas and Moazzami, Ali and Bergquist, Jonas and Olszewski, Pawel K. and Schioth, Helgi B. | ||||||
|---|---|---|---|---|---|---|---|
| Title: | The polyamine transporter Slc18b1(VPAT) is important for both short and long time memory and for regulation of polyamine content in the brain | ||||||
| Series Name/Journal: | PLoS Genetics | ||||||
| Year of publishing : | 2019 | ||||||
| Volume: | 15 | ||||||
| Page range: | 1-30 | ||||||
| Number of Pages: | 30 | ||||||
| Publisher: | PLoS | ||||||
| ISSN: | 1553-7390 | ||||||
| Language: | English | ||||||
| Publication Type: | Research article | ||||||
| Article category: | Scientific peer reviewed | ||||||
| Version: | Published version | ||||||
| Copyright: | Creative Commons: Attribution 4.0 | ||||||
| Full Text Status: | Public | ||||||
| Subjects: | (A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 301 Basic Medicine > Neurosciences | ||||||
| Keywords: | polyamine transporters, Slc18b1, nerve cells, memory, learning, Long Term Potentiation | ||||||
| URN:NBN: | urn:nbn:se:slu:epsilon-p-104555 | ||||||
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-104555 | ||||||
| Additional ID: |
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| ID Code: | 16714 | ||||||
| Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
| Department: | (NL, NJ) > Department of Molecular Sciences | ||||||
| Deposited By: | SLUpub Connector | ||||||
| Deposited On: | 30 Mar 2020 08:28 | ||||||
| Metadata Last Modified: | 30 Mar 2020 08:28 |
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