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Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen

Kucukoglu, Melis and Chaabouni, Salma and Zheng, Bo and Mahonen, Ari Pekka and Helariutta, Yka and Nilsson, Ove (2020). Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen. New Phytologist. 226 , 75-85
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Abstract

The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (ESR)-RELATED (CLE) peptide ligands in connection with their receptors are important players in cell-to-cell communications in plants. Here, we investigated the function of the Populus CLV3/ESR-RELATED 47 (PttCLE47) gene during secondary growth and wood formation in hybrid aspen (Populus tremula x tremuloides) using an RNA interference (RNAi) approach. Expression of PttCLE47 peaks in the vascular cambium. Silencing of the PttCLE47 gene expression affected lateral expansion of stems and decreased apical height growth and leaf size. In particular, PttCLE47 RNAi trees exhibited a narrower secondary xylem zone with less xylem cells/cell file. The reduced radial growth phenotype also correlated with a reduced number of cambial cell layers. In agreement with these results, expression of several cambial regulator genes was downregulated in the stems of the transgenic trees in comparison with controls. Altogether, these results suggest that the PttCLE47 gene is a major positive regulator of cambial activity in hybrid aspen, mainly promoting the production of secondary xylem. Furthermore, in contrast to previously characterized CLE genes expressed in the wood-forming zone, PttCLE47 appears to be active at its site of expression.

Authors/Creators:Kucukoglu, Melis and Chaabouni, Salma and Zheng, Bo and Mahonen, Ari Pekka and Helariutta, Yka and Nilsson, Ove
Title:Peptide encoding Populus CLV3/ESR-RELATED 47 (PttCLE47) promotes cambial development and secondary xylem formation in hybrid aspen
Year of publishing :2020
Volume:226
Page range:75-85
Number of Pages:11
Publisher:Wiley
ISSN:0028-646X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:cambium, CLE peptides, Populus, PttCLE47, secondary growth and development, wood formation
URN:NBN:urn:nbn:se:slu:epsilon-p-103476
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-103476
Additional ID:
Type of IDID
DOI10.1111/nph.16331
Web of Science (WoS)000504373000001
ID Code:16792
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:31 Mar 2020 09:32
Metadata Last Modified:22 Aug 2020 22:54

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