Home About Browse Search
Svenska


Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post-mortem examination

Blomqvist, M.A. and Ley, Cecilia and Karlsson, H.K. and Hanson, Jeanette (2020). Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post-mortem examination. Journal of Comparative Pathology. 176 , 116-121
[Journal article]

[img] PDF - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

730kB

Abstract

Hypophysitis has been reported occasionally in dogs, with most cases resembling primary lymphocytic hypo-physitis in man. Although it is generally assumed that lymphocytes are not present normally in the canine pi-tuitary gland, few studies have investigated this hypothesis. However, lymphocytes are recognized in thepituitary gland of people and horses without signs of pituitary disease. It is unknown to what degree lympho-cyte infiltration of the pituitary gland might occur as an incidental finding in dogs. The aim of the present studywas to investigate the presence and distribution of lymphocytes in the pituitary gland of dogs without clinicalsuspicion of pituitary disease. Twenty dogs were subjected to routine necropsy examination. Formalin-fixedand paraffin wax-embedded sections of pituitary were stained with haematoxylin and eosin (HE) or subjectedto immunohistochemistry (IHC) using primary antibodies specific for the T-cell marker CD3 and the B-cellmarker CD79a. The number of CD3+ and CD79a+ cells per area unit (CPA) was determined for differentpituitary regions. Two dogs had extensive neoplastic lesions in the pituitary gland and were excluded fromanalysis. In the remaining 18 dogs, occasional scattered CD3+cells were found in the pituitary gland. Therewas a significant difference in CD3+CPA between pituitary regions (P¼0.001). The highest CD3+CPA wasfound in the pars tuberalis (median 41.3 cells/mm2, interquartile range 20.9e50.5 cells/mm2). In six of the 18dogs (33%), CD79a+ cells were detected in small number (median total cell number 0 cells/section, interquar-tile range 0e1.0 cells/section). This study shows that T cell, and fewer B cells, may be found in the pituitarygland of dogs without clinical suspicion of pituitary disease. Regional difference in T-cell density, with the high-est CD3+CPA in the pars tuberalis, may imply regional immunoregulatory functions in the canine pituitarygland.

Authors/Creators:Blomqvist, M.A. and Ley, Cecilia and Karlsson, H.K. and Hanson, Jeanette
Title:Presence of CD3+ and CD79a+ lymphocytes in the pituitary gland of dogs at post-mortem examination
Year of publishing :2020
Volume:176
Page range:116-121
Number of Pages:6
Publisher:Elsevier
ISSN:0021-9975
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:B cell, dogs, hypophysitis, T cell
URN:NBN:urn:nbn:se:slu:epsilon-p-104960
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-104960
Additional ID:
Type of IDID
DOI10.1016/j.jcpa.2020.02.010
ID Code:16799
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
(VH) > Dept. of Clinical Sciences
Deposited By: SLUpub Connector
Deposited On:31 Mar 2020 10:23
Metadata Last Modified:31 Mar 2020 10:23

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits