Aboshady, Hadeer M. and Mandonnet, Nathalie and Felicite, Yoann and Hira, Julien and Fourcot, Aurore and Barbier, Claude and Johansson, Anna Maria and Jonas, Elisabeth and Bambou, Jean-Christophe
(2020).
Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection.
Veterinary Research. 51
, 1-12
[Journal article]
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Abstract
Gastrointestinal nematode (GIN) infections are one of the major constraints for grazing sheep and goat production worldwide. Genetic selection for resistant animals is a promising control strategy. Whole-transcriptome analysis via RNA-sequencing (RNA-seq) provides knowledge of the mechanisms responsible for complex traits such as resistance to GIN infections. In this study, we used RNA-seq to monitor the dynamics of the response of the abomasal mucosa of Creole goat kids infected with Haemonchus contortus by comparing resistant and susceptible genotypes. A total of 8 cannulated kids, 4 susceptible and 4 resistant to GIN, were infected twice with 10 000 L3 H. contortus. During the second infection, abomasal mucosal biopsies were collected at 0, 8, 15 and 35 days post-infection (dpi) from all kids for RNA-seq analysis. The resistant animals showed early activation of biological processes related to the immune response. The top 20 canonical pathways of differentially expressed genes for different comparison showed activation of the immune response through many relevant pathways including the Th1 response. Interestingly, our results showed a simultaneous time series activation of Th2 related genes in resistant compared to susceptible kids.
Authors/Creators: | Aboshady, Hadeer M. and Mandonnet, Nathalie and Felicite, Yoann and Hira, Julien and Fourcot, Aurore and Barbier, Claude and Johansson, Anna Maria and Jonas, Elisabeth and Bambou, Jean-Christophe | ||||||
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Title: | Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 51 | ||||||
Page range: | 1-12 | ||||||
Number of Pages: | 12 | ||||||
Publisher: | BMC | ||||||
ISSN: | 0928-4249 | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science | ||||||
Keywords: | Gastrointestinal nematode (GIN) infections, sheep, goats, genetic selection, resistance | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-105155 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105155 | ||||||
Additional ID: |
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ID Code: | 16856 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Dept. of Animal Breeding and Genetics | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 15 Apr 2020 14:44 | ||||||
Metadata Last Modified: | 15 Jan 2021 19:02 |
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