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Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection

Aboshady, Hadeer M. and Mandonnet, Nathalie and Felicite, Yoann and Hira, Julien and Fourcot, Aurore and Barbier, Claude and Johansson, Anna Maria and Jonas, Elisabeth and Bambou, Jean-Christophe (2020). Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection. Veterinary Research. 51 , 1-12
[Journal article]

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Abstract

Gastrointestinal nematode (GIN) infections are one of the major constraints for grazing sheep and goat production worldwide. Genetic selection for resistant animals is a promising control strategy. Whole-transcriptome analysis via RNA-sequencing (RNA-seq) provides knowledge of the mechanisms responsible for complex traits such as resistance to GIN infections. In this study, we used RNA-seq to monitor the dynamics of the response of the abomasal mucosa of Creole goat kids infected with Haemonchus contortus by comparing resistant and susceptible genotypes. A total of 8 cannulated kids, 4 susceptible and 4 resistant to GIN, were infected twice with 10 000 L3 H. contortus. During the second infection, abomasal mucosal biopsies were collected at 0, 8, 15 and 35 days post-infection (dpi) from all kids for RNA-seq analysis. The resistant animals showed early activation of biological processes related to the immune response. The top 20 canonical pathways of differentially expressed genes for different comparison showed activation of the immune response through many relevant pathways including the Th1 response. Interestingly, our results showed a simultaneous time series activation of Th2 related genes in resistant compared to susceptible kids.

Authors/Creators:Aboshady, Hadeer M. and Mandonnet, Nathalie and Felicite, Yoann and Hira, Julien and Fourcot, Aurore and Barbier, Claude and Johansson, Anna Maria and Jonas, Elisabeth and Bambou, Jean-Christophe
Title:Dynamic transcriptomic changes of goat abomasal mucosa in response to Haemonchus contortus infection
Year of publishing :2020
Volume:51
Page range:1-12
Number of Pages:12
Publisher:BMC
ISSN:0928-4249
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 403 Veterinary Science > Clinical Science
Keywords:Gastrointestinal nematode (GIN) infections, sheep, goats, genetic selection, resistance
URN:NBN:urn:nbn:se:slu:epsilon-p-105155
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105155
Additional ID:
Type of IDID
DOI10.1186/s13567-020-00768-y
Web of Science (WoS)000521026000001
ID Code:16856
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Animal Breeding and Genetics
Deposited By: SLUpub Connector
Deposited On:15 Apr 2020 14:44
Metadata Last Modified:15 Apr 2020 14:44

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