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Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers

Ouni, Rim and Zborowska, Anna and Sehic, Jasna and Choulak, Sarra and Inaki Hormaza, J. and Gustavsson, Larisa and Mars, Messaoud (2020). Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers. Horticultural plant journal. 6 :2 , 61-70
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Abstract

Growing pear has a long tradition in Tunisia, and numerous local cultivars possessing an excellent adaptability and resilience potential to climatic variation are present. This large adaptability is associated with an important genetic diversity, which is threatened to erosion. Appropriate measures have to be taken in order to properly evaluate and conserve this local material. Microsatellite markers were used to assess the level of genetic diversity among Tunisian pear germplasm, and compare it with some European varieties and wild pear species. 61 pear accessions representing eight groups (six groups from Tunisia, one from Northern Europe and another group composed of wild pear) have been genotyped using SSR markers derived from apple and pear. The pear accessions showed a significant polymorphism and 95 polymorphic alleles were found. The number of alleles per locus varied from 5 for CH04e03 locus to 14 for CH01d09 locus with an average of 9.4 alleles per locus. Moreover, the mean gene diversity (H-e) per locus ranged from 0.192 to 0.752. Genetic distance values and cluster analyses revealed high genetic similarities among the Tunisian groups. Factorial correspondence analysis (FCA) categorized the accessions into three independent groups where Tunisian local accessions agglomerated together distantly from European and wild pear accessions. Additionally, UPGMA dendrogram grouped accessions into two clusters, confirmed thereafter by the Bayesian model-based Structure analysis. The results showed 16 putative triploid accessions found in the local germplasm. This study provides valuable information to develop strategies of local pear conservation and use.

Authors/Creators:Ouni, Rim and Zborowska, Anna and Sehic, Jasna and Choulak, Sarra and Inaki Hormaza, J. and Gustavsson, Larisa and Mars, Messaoud
Title:Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers
Year of publishing :2020
Volume:6
Number:2
Page range:61-70
Number of Pages:10
Publisher:Elsevier
Associated Programs and Other Stakeholders:Z - SLU - Libray > Odla mera
ISSN:2095-9885
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Horticulture
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Botany
Keywords:Pyrus communis, molecular marker, genetic diversity, local accession
URN:NBN:urn:nbn:se:slu:epsilon-p-105295
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105295
Additional ID:
Type of IDID
DOI10.1016/j.hpj.2020.03.003
Web of Science (WoS)000525290800001
ID Code:16920
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:15 May 2020 12:39
Metadata Last Modified:23 Aug 2020 20:33

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