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Cross-Kingdom RNAi of Pathogen Effectors Leads to Quantitative Adult Plant Resistance in Wheat

Schaefer, Luisa Katharina and Parlange, Francis and Buchmann, Gabriele and Jung, Esther and Wehrli, Andreas and Herren, Gerhard and Mueller, Marion Claudia and Stehlin, Jonas and Schmid, Roman and Wicker, Thomas and Keller, Beat and Bourras, Salim (2020). Cross-Kingdom RNAi of Pathogen Effectors Leads to Quantitative Adult Plant Resistance in Wheat. Frontiers in Plant Science. 11 , 253 , 1-13
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Abstract

Cross-kingdom RNA interference (RNAi) is a biological process allowing plants to transfer small regulatory RNAs to invading pathogens to trigger the silencing of target virulence genes. Transient assays in cereal powdery mildews suggest that silencing of one or two effectors could lead to near loss of virulence, but evidence from stable RNAi lines is lacking. We established transient host-induced gene silencing (HIGS) in wheat, and demonstrate that targeting an essential housekeeping gene in the wheat powdery mildew pathogen (Blumeria graminis f. sp. tritici) results in significant reduction of virulence at an early stage of infection. We generated stable transgenic RNAi wheat lines encoding a HIGS construct simultaneously silencing three B.g. tritici effectors including SvrPm3(a1/f1), a virulence factor involved in the suppression of the Pm3 powdery mildew resistance gene. We show that all targeted effectors are effectively downregulated by HIGS, resulting in reduced fungal virulence on adult wheat plants. Our findings demonstrate that stable HIGS of effector genes can lead to quantitative gain of resistance without major pleiotropic effects in wheat.

Authors/Creators:Schaefer, Luisa Katharina and Parlange, Francis and Buchmann, Gabriele and Jung, Esther and Wehrli, Andreas and Herren, Gerhard and Mueller, Marion Claudia and Stehlin, Jonas and Schmid, Roman and Wicker, Thomas and Keller, Beat and Bourras, Salim
Title:Cross-Kingdom RNAi of Pathogen Effectors Leads to Quantitative Adult Plant Resistance in Wheat
Year of publishing :2020
Volume:11
Article number:253
Number of Pages:13
Publisher:Frontiers Media
ISSN:1664-462X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:cross-kingdom RNAi, ck-RNAi, host-induced gene silencing, HIGS, ribonuclease-like effectors, effectors, Blumeria graminis, wheat
URN:NBN:urn:nbn:se:slu:epsilon-p-105283
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105283
Additional ID:
Type of IDID
DOI10.3389/fpls.2020.00253
Web of Science (WoS)000525204100001
ID Code:16924
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:18 May 2020 12:57
Metadata Last Modified:05 Aug 2020 17:29

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