Tzelepis, Georgios and Persson Hodén, Kristian and Fogelqvist, Johan and Åsman, Anna and Vetukuri, Ramesh and Dixelius, Christina
(2020).
Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans.
Frontiers in Microbiology. 11
, 252
, 1-12
[Journal article]
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- Published Version
Available under License Creative Commons Attribution. 4MB |
Abstract
The potato late blight pathogen Phytophthora infestans has both an asexual and a sexual mode of reproduction. In Scandinavia, the pathogen is reproducing sexually on a regular basis, whereas clonal lineages dominate in other geographical regions. This study aimed at elucidating events or key genes underlying this difference in sexual behavior. First, the transcriptomes of eight strains, known as either clonal or sexual, were compared during early stages of mating. Principal component analysis (PCA) divided the samples in two clusters A and B and a clear grouping of the mating samples together with the A1 mating type parents was observed. Induction of genes encoding DNA adenine N6-methylation (6mA) methyl-transferases clearly showed a bias toward the cluster A. In contrast, the Avrblb2 effector gene family was highly induced in most of the mating samples and was associated with cluster B in the PCA, similarly to genes coding for acetyl-transferases, which play an important role in RXLR modification prior to secretion. Avrblb2 knock-down strains displayed a reduction in virulence and oospore formation, suggesting a role during the mating process. In conclusion, a number of gene candidates important for the reproductive processes were revealed. The results suggest a possible epigenetic influence and involvement of specific RXLR effectors in mating-related processes.
Authors/Creators: | Tzelepis, Georgios and Persson Hodén, Kristian and Fogelqvist, Johan and Åsman, Anna and Vetukuri, Ramesh and Dixelius, Christina | ||||||
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Title: | Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans | ||||||
Series Name/Journal: | Frontiers in Microbiology | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 11 | ||||||
Article number: | 252 | ||||||
Number of Pages: | 12 | ||||||
Publisher: | Frontiers | ||||||
ISSN: | 1664-302X | ||||||
Language: | English | ||||||
Publication Type: | Journal article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402) (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science | ||||||
Keywords: | Avrblb2, epigenetics, heterothallism, mating, ploidy | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-105301 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105301 | ||||||
Additional ID: |
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ID Code: | 16930 | ||||||
Faculty: | NJ - Fakulteten för naturresurser och jordbruksvetenskap | ||||||
Department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 02 Jun 2020 07:21 | ||||||
Metadata Last Modified: | 31 Aug 2020 15:29 |
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