Home About Browse Search
Svenska


Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans

Tzelepis, Georgios and Persson Hodén, Kristian and Fogelqvist, Johan and Åsman, Anna and Vetukuri, Ramesh and Dixelius, Christina (2020). Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans. Frontiers in Microbiology. 11 , 252 , 1-12
[Journal article]

[img] PDF - Published Version
Available under License Creative Commons Attribution.

4MB

Abstract

The potato late blight pathogen Phytophthora infestans has both an asexual and a sexual mode of reproduction. In Scandinavia, the pathogen is reproducing sexually on a regular basis, whereas clonal lineages dominate in other geographical regions. This study aimed at elucidating events or key genes underlying this difference in sexual behavior. First, the transcriptomes of eight strains, known as either clonal or sexual, were compared during early stages of mating. Principal component analysis (PCA) divided the samples in two clusters A and B and a clear grouping of the mating samples together with the A1 mating type parents was observed. Induction of genes encoding DNA adenine N6-methylation (6mA) methyl-transferases clearly showed a bias toward the cluster A. In contrast, the Avrblb2 effector gene family was highly induced in most of the mating samples and was associated with cluster B in the PCA, similarly to genes coding for acetyl-transferases, which play an important role in RXLR modification prior to secretion. Avrblb2 knock-down strains displayed a reduction in virulence and oospore formation, suggesting a role during the mating process. In conclusion, a number of gene candidates important for the reproductive processes were revealed. The results suggest a possible epigenetic influence and involvement of specific RXLR effectors in mating-related processes.

Authors/Creators:Tzelepis, Georgios and Persson Hodén, Kristian and Fogelqvist, Johan and Åsman, Anna and Vetukuri, Ramesh and Dixelius, Christina
Title:Dominance of Mating Type A1 and Indication of Epigenetic Effects During Early Stages of Mating in Phytophthora infestans
Year of publishing :2020
Volume:11
Article number:252
Number of Pages:12
Publisher:Frontiers
ISSN:1664-302X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Genetics (medical genetics to be 30107 and agricultural genetics to be 40402)
(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Avrblb2, epigenetics, heterothallism, mating, ploidy
URN:NBN:urn:nbn:se:slu:epsilon-p-105301
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105301
Additional ID:
Type of IDID
DOI10.3389/fmicb.2020.00252
Web of Science (WoS)000524586700001
ID Code:16930
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:02 Jun 2020 07:21
Metadata Last Modified:31 Aug 2020 15:29

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits