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Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germination

Kumar, Dhananjay and Das, P. K. and Sarmah, B. K. (2020). Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germination. Biologia Plantarum. 64 , 244-252
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Abstract

Flood in rice fields at the time of seed sowing and early seedling establishment causes extensive crop loss due to the inability of the seeds to tolerate and overcome submergence. In the present study, rice genotypes from Assam, India tolerant to hypoxia during germination were identified through systematic screening of deep-water rice genotype collection from flood-prone ecosystem of Brahmaputra river valley. The difference in tolerance to hypoxia during germination within the species provides scope for identification of novel hypoxia-responsive factors involved in tolerance as mechanism of tolerance may not be conserved across tolerant gennplasms. Tolerant genotypes were further subjected to physiological and molecular characterization. Growth rate kinetics in tolerant genotypes new Rangdhakekua bao (RKB) and cultivar Khao Hlan On (KHO) from International Rice Research Institute (IRRI) used as positive control exhibited stronger escape strategy under hypoxic condition compared to sensitive genotype IR-64 (negative control). Activities of a-amylase and pyruvate decarboxylase were significantly higher in RKB and KHO than in IR-64, while no significant difference was observed in the alcohol dehydrogenase activity. Reverse transcription quantitative PCR confirmed increased amounts of transcripts of sucrose nonfermenting I related protein kinase, myeloblastosis-related protein S1, rice amylase 3D, and trehalose phosphate phosphatase 7 genes, which are known to be involved in hypoxia signaling cascade. Besides, transcription factors (TFs) like ethylene responce factors 71 and 63, ethylene insensitive 3-like la and proteins like cxpansins A7 and A2, which are involved in cell elongation had also significantly higher amounts in RKB compared to IR64. Additional factors that include TFs like ERF71 and FRF63 that shows perturbation at transcription even within tolerant genotypes might constitute the genotype-specific regulation, evolved as a part of its adaptive mechanism to survive under submerged conditions.

Authors/Creators:Kumar, Dhananjay and Das, P. K. and Sarmah, B. K.
Title:Identification of novel hypoxia-responsive factors in deep-water rice conferring tolerance to flood during germination
Year of publishing :2020
Volume:64
Page range:244-252
Number of Pages:9
Publisher:Kluwer
ISSN:0006-3134
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:alpha-amylase, anaerobic germination, Oryza sativa, pyruvate decarboxylase, transcription factors
URN:NBN:urn:nbn:se:slu:epsilon-p-105291
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105291
Additional ID:
Type of IDID
DOI10.32615/bp.2019.128
Web of Science (WoS)000521969800001
ID Code:16954
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:01 Jun 2020 12:47
Metadata Last Modified:05 Aug 2020 17:26

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