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Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood

Ma, Biao and Li, Jiali and Chen, Kai and Yu, Xiaoping and Sun, Chuanxin and Zhang, Mingzhou (2020). Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood. Foods. 9 , 278 , 1-15
[Journal article]

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Abstract

Foodborne pathogens can cause foodborne illness. In reality, one food sample may carry more than one pathogen. A rapid, sensitive, and multiple target method for bacteria detection is crucial in food safety. For the simultaneous detection of Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella Enteritidis, multi-objective recombinase polymerase amplification (RPA) combined with a lateral flow dipstick (LFD) was developed in this study. The whole process, including amplification and reading, can be completed in 15 min at 37 degrees C. The detection limits were 2.6 x 10(1) CFU/mL for Staphylococcus aureus, 7.6 x 10(1) CFU/mL for Vibrio parahaemolyticus, and 1.29 x 10(1) CFU/mL for Salmonella Enteritidis. Moreover, colored signal intensities on test lines were measured by a test strip reader to achieve quantitative detection for Staphylococcus aureus (R-2 = 0.9903), Vibrio parahaemolyticus (R-2 = 0.9928), and Salmonella Enteritidis (R-2 = 0.9945). In addition, the method demonstrated good recoveries (92.00%-107.95%) in the testing of spiked food samples. Therefore, the multiplex LFD-RPA assay is a feasible method for the rapid, sensitive, and quantitative detection of bacterial pathogens in seafood.

Authors/Creators:Ma, Biao and Li, Jiali and Chen, Kai and Yu, Xiaoping and Sun, Chuanxin and Zhang, Mingzhou
Title:Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood
Year of publishing :2020
Volume:9
Article number:278
Number of Pages:15
Publisher:MDPI
ISSN:2304-8158
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Food Science
Keywords:recombinase polymerase amplification, lateral flow dipstick, multiplex detection, quantitative detection, Staphylococcus aureus, Vibrio parahaemolyticus, Salmonella Enteritidis
URN:NBN:urn:nbn:se:slu:epsilon-p-105387
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105387
Additional ID:
Type of IDID
DOI10.3390/foods9030278
Web of Science (WoS)000526529800122
ID Code:16958
Faculty:NJ - Fakulteten för naturresurser och jordbruksvetenskap
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Deposited By: SLUpub Connector
Deposited On:08 May 2020 08:37
Metadata Last Modified:08 May 2020 08:37

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