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Production of human heme-binding proteins in plants for potential pharmaceutical and nutritional uses

Carlsson, Magnus (2020). Production of human heme-binding proteins in plants for potential pharmaceutical and nutritional uses. Diss. (sammanfattning/summary) Sveriges lantbruksuniv., Acta Universitatis Agriculturae Sueciae, 1652-6880 ; 2020:34
ISBN 978-91-7760-586-7
eISBN 978-91-7760-587-4
[Doctoral thesis]

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Abstract

Plants have a long history as a source of nutrition and medication. In the last few decades, interest has also emerged in using plants as a production system for protein pharmaceuticals, in part due to possible scale-up- and safety-benefits with this method of production. One class of protein pharmaceuticals for which the use of a plant production system might be particularly interesting is hemoglobin-based oxygen carriers (HBOC’s), a type of pharmaceutical intended to function as an alternative to donated blood in clinical situations. HBOC’s face many safety and production challenges, that have so far prevented widespread use, and production in plants could perhaps help towards solving these problems. In this thesis, we aimed to express and characterize proteins of interest with the goal of using them in oxygen therapeutics, using transient expression in Nicotiana benthamiana. The expressed recombinant proteins were based on human myoglobin (Mb), fetal hemoglobin (HbF) and α1-microglobulin (A1M). The proteins were successfully expressed in tobacco leaves and the purified proteins displayed their respective functional activity. The plant expression system thus showed its efficacy, and was capable of supplying the critical heme for Mb and Hb production, something that may also have implications for using plants as a source of nutritional iron. While more work is needed, the results help to show the feasibility of using heme-binding proteins produced in plants for pharmaceutical uses, something that was also underlined by animal- and cell-tests.

Authors/Creators:Carlsson, Magnus
Title:Production of human heme-binding proteins in plants for potential pharmaceutical and nutritional uses
Year of publishing :2020
Number:2020:34
Number of Pages:62
Publisher:Department of Plant Breeding, Swedish University of Agricultural Sciences
ISBN for printed version:978-91-7760-586-7
ISBN for electronic version:978-91-7760-587-4
ISSN:1652-6880
Language:English
Publication Type:Doctoral thesis
Article category:Other scientific
Version:Published version
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 404 Agricultural Biotechnology > Plant Biotechnology
(A) Swedish standard research categories 2011 > 3 Medical and Health Sciences > 304 Medical Biotechnology > Other Medical Biotechnology
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:Molecular farming, heterologous expression, transient expression, oxygen therapeutic, HBOC, hemoglobin, myoglobin, α1-microglobulin, A1M, XTEN
URN:NBN:urn:nbn:se:slu:epsilon-p-105512
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105512
ID Code:16982
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:15 May 2020 14:20
Metadata Last Modified:09 Sep 2020 14:18

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