under controlled and field conditions
Jäderlund, Lotta
(2008).
Fates and impacts of the genetically modified plant growth-promoting bacterium Pseudomonas fluorescens SBW25.
Diss. (sammanfattning/summary)
Uppsala :
Sveriges lantbruksuniv.,
Acta Universitatis Agriculturae Sueciae, 1652-6880
; 2008:15
ISBN 978-91-859-1348-0
[Doctoral thesis]
![]() |
PDF
4MB |
Abstract
Plant growth-promoting bacteria may be used in agriculture to minimize the utilization of chemical pesticides and fertilizers. This thesis studies one plant growth-promoting bacterial strain, Pseudomonas fluorescens SBW25, and its interactions with winter wheat, potato and tomato in both field and laboratory conditions. This bacterium was tagged with a novel marker gene cassette containing gfp (green fluorescent protein), luxAB (bacterial bioluminescence) and telABkilA (potassium tellurite oxide resistance) and the resulting strain SBW25::tgl was released in the first field trial with a genetically modified microorganism in Sweden. Bacterial numbers on wheat plants and in soil were determined by selective plating on media containing potassium tellurite oxide. The impact of SBW25::tgl on wheat plant growth was measured using plant length and weight, and possible effects on resident bacterial and fungal microflora was evaluated throughout the 8 months sampling period using terminal restriction fragment length polymorphism (T-RFLP). The GMM was found in high numbers on all plant parts throughout the 8 months period, but only minor impacts were found on native microflora due to bacterial (GMM or wild-type) inoculation. SBW25 was also evaluated as a biocontrol agent towards the phytopathogenic bacterium Ralstonia solanacearum on tomato and potato plants. By using gfp-tagged R. solanacearum and red fluorescent protein (rfp) -tagged SBW25 it was possible to distinguish these two strains on plant surfaces using microscopic techniques. Plate counting, flow cytometry and luminometry were used to monitor the strains on plants. Some biocontrol effect of SBW25 was detected on tomato plants. The interactions of SBW25 and arbuscular mycorrhizal (AM) fungi Glomus mosseae and G. intraradices were also studied. Very specific interactions were found, and also synergistic biocontrol effects suggesting that a consortium of microorganisms might be a better choice when applied as plant growth-promoters or biocontrol agents.
Authors/Creators: | Jäderlund, Lotta | ||||
---|---|---|---|---|---|
Title: | Fates and impacts of the genetically modified plant growth-promoting bacterium Pseudomonas fluorescens SBW25 | ||||
Subtitle: | under controlled and field conditions | ||||
Year of publishing : | 2008 | ||||
Number: | 2008:15 | ||||
Number of Pages: | 53 | ||||
Papers/manuscripts: |
| ||||
Place of Publication: | Uppsala | ||||
ISBN for printed version: | 978-91-859-1348-0 | ||||
ISSN: | 1652-6880 | ||||
Language: | English | ||||
Publication Type: | Doctoral thesis | ||||
Full Text Status: | Public | ||||
Agrovoc terms: | pseudomonas fluorescens, pseudomonas solanacearum, plant growth substances, genetically modified microorganisms, triticum, winter crops, lycopersicon esculentum, solanum tuberosum, genetic markers, plant response, monographella nivalis, biological control, mycorrhizae, field experimentation, sweden | ||||
Keywords: | plant growth-promoting bacteria (PGPB), Pseudomonas fluorescens SBW25, marker genes, field trial, arbuscular mycorhizal fungi, Microdochium nivale, Ralstonia solanacearum, terminal restriction fragment length polymorphism (T-RFLP) | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-2129 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-2129 | ||||
ID Code: | 1704 | ||||
Department: | (NL, NJ) > Dept. of Microbiology (until 161231) | ||||
Deposited By: | Lotta Jäderlund | ||||
Deposited On: | 12 Mar 2008 00:00 | ||||
Metadata Last Modified: | 02 Dec 2014 10:13 |
Repository Staff Only: item control page