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Plant based production of myoglobin - a novel source of the muscle heme-protein

Kanagarajan, Selvaraju and Carlsson, Magnus and Bülow, Leif and Zhu, Li-Hua (2020). Plant based production of myoglobin - a novel source of the muscle heme-protein. Scientific Reports. 10 , 920 , 1-9
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Abstract

Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. in this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. the results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.

Authors/Creators:Kanagarajan, Selvaraju and Carlsson, Magnus and Bülow, Leif and Zhu, Li-Hua
Title:Plant based production of myoglobin - a novel source of the muscle heme-protein
Year of publishing :2020
Volume:10
Article number:920
Number of Pages:10
Publisher:Nature Research
ISSN:2045-2322
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology
Keywords:plant sciences, molecular biology, biotechnology, plant biotechnology
URN:NBN:urn:nbn:se:slu:epsilon-p-105690
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105690
Additional ID:
Type of IDID
DOI10.1038/s41598-020-57565-y
ID Code:17096
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Deposited By: SLUpub Connector
Deposited On:10 Jun 2020 12:17
Metadata Last Modified:02 Aug 2020 21:46

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