Carlsson, Magnus and Kanagarajan, Selvaraju and Bülow, Leif and Zhu, Li-Hua
(2020).
Plant based production of myoglobin - a novel source of the muscle heme-protein.
Scientific Reports. 10
, 920
, 1-9
[Journal article]
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Available under License Creative Commons Attribution. 2MB |
Abstract
Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nutritional source of bioavailable iron. in this study, we have successfully produced human myoglobin in the leaves of Nicotiana benthamiana by transient expression using a viral vector delivered by Agrobacterium tumefaciens. Analyses confirmed that heme was incorporated and the protein was functional, with observed properties consistent with those of native myoglobins. A relatively high degree of purity could be achieved with low cost methods. the results show the high potential of plants as a production platform for heme proteins, a group of proteins of interest for iron nutrition applications and possible future pharmaceutical development.
Authors/Creators: | Carlsson, Magnus and Kanagarajan, Selvaraju and Bülow, Leif and Zhu, Li-Hua | ||||
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Title: | Plant based production of myoglobin - a novel source of the muscle heme-protein | ||||
Series Name/Journal: | Scientific Reports | ||||
Year of publishing : | 2020 | ||||
Volume: | 10 | ||||
Article number: | 920 | ||||
Number of Pages: | 10 | ||||
Publisher: | Nature Research | ||||
ISSN: | 2045-2322 | ||||
Language: | English | ||||
Publication Type: | Journal article | ||||
Article category: | Scientific peer reviewed | ||||
Version: | Published version | ||||
Copyright: | Creative Commons: Attribution 4.0 | ||||
Full Text Status: | Public | ||||
Subjects: | (A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Biochemistry and Molecular Biology | ||||
Keywords: | plant sciences, molecular biology, biotechnology, plant biotechnology | ||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-105690 | ||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105690 | ||||
Additional ID: |
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ID Code: | 17096 | ||||
Faculty: | LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap | ||||
Department: | (LTJ, LTV) > Department of Plant Breeding (from 130101) | ||||
Deposited By: | SLUpub Connector | ||||
Deposited On: | 10 Jun 2020 12:17 | ||||
Metadata Last Modified: | 30 Oct 2020 10:16 |
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