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The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalis

Li, Zhiqiang and Peirasmaki, Dimitra and Svaerd, Staffan and Åbrink, Magnus (2020). The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalis. Cells. 9 , 925 , 1-19
[Journal article]

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Abstract

Mast cells have been shown to affect the control of infections with the protozoan parasite Giardia intestinalis. Recently, we demonstrated that Giardia excretory-secretory proteins inhibited the activity of the connective tissue mast cell-specific protease chymase. To study the potential role of the chymase mouse mast cell protease (mMCP)-4 during infections with Giardia, mMCP-4(+/+) and mMCP-4(-/-) littermate mice were gavage-infected with G. intestinalis trophozoites of the human assemblage B isolate GS. No significant changes in weight gain was observed in infected young (approximate to 10 weeks old) mMCP-4(-/-) and mMCP-4(+/+) littermate mice. In contrast, infections of mature adult mice (>18 weeks old) caused significant weight loss as compared to uninfected control mice. We detected a more rapid weight loss in mMCP-4(-/-) mice as compared to littermate mMCP-4(+/+) mice. Submucosal mast cell and granulocyte counts in jejunum increased in the infected adult mMCP-4(-/-) and mMCP-4(+/+) mice. This increase was correlated with an augmented intestinal trypsin-like and chymotrypsin-like activity, but the myeloperoxidase activity was constant. Infected mice showed a significantly lower intestinal neutrophil elastase (NE) activity, and in vitro, soluble Giardia proteins inhibited human recombinant NE. Serum levels of IL-6 were significantly increased eight and 13 days post infection (dpi), while intestinal IL-6 levels showed a trend to significant increase 8 dpi. Strikingly, the lack of mMCP-4 resulted in significantly less intestinal transcriptional upregulation of IL-6, TNF-alpha, IL-25, CXCL2, IL-2, IL-4, IL-5, and IL-10 in the Giardia-infected mature adult mice, suggesting that chymase may play a regulatory role in intestinal cytokine responses.

Authors/Creators:Li, Zhiqiang and Peirasmaki, Dimitra and Svaerd, Staffan and Åbrink, Magnus
Title:The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalis
Year of publishing :2020
Volume:9
Article number:925
Number of Pages:19
Publisher:MDPI
ISSN:2073-4409
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
Keywords:mast cell, chymase, mMCP-4, infection, Giardia intestinalis
URN:NBN:urn:nbn:se:slu:epsilon-p-106502
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106502
Additional ID:
Type of IDID
DOI10.3390/cells9040925
Web of Science (WoS)000535559500136
ID Code:17192
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Department of Biomedical Science and Veterinary Public Health
Deposited By: SLUpub Connector
Deposited On:22 Jun 2020 13:40
Metadata Last Modified:22 Jun 2020 13:40

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