Li, Zhiqiang and Peirasmaki, Dimitra and Svaerd, Staffan and Åbrink, Magnus
(2020).
The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalis.
Cells. 9
, 925
, 1-19
[Research article]
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Abstract
Mast cells have been shown to affect the control of infections with the protozoan parasite Giardia intestinalis. Recently, we demonstrated that Giardia excretory-secretory proteins inhibited the activity of the connective tissue mast cell-specific protease chymase. To study the potential role of the chymase mouse mast cell protease (mMCP)-4 during infections with Giardia, mMCP-4(+/+) and mMCP-4(-/-) littermate mice were gavage-infected with G. intestinalis trophozoites of the human assemblage B isolate GS. No significant changes in weight gain was observed in infected young (approximate to 10 weeks old) mMCP-4(-/-) and mMCP-4(+/+) littermate mice. In contrast, infections of mature adult mice (>18 weeks old) caused significant weight loss as compared to uninfected control mice. We detected a more rapid weight loss in mMCP-4(-/-) mice as compared to littermate mMCP-4(+/+) mice. Submucosal mast cell and granulocyte counts in jejunum increased in the infected adult mMCP-4(-/-) and mMCP-4(+/+) mice. This increase was correlated with an augmented intestinal trypsin-like and chymotrypsin-like activity, but the myeloperoxidase activity was constant. Infected mice showed a significantly lower intestinal neutrophil elastase (NE) activity, and in vitro, soluble Giardia proteins inhibited human recombinant NE. Serum levels of IL-6 were significantly increased eight and 13 days post infection (dpi), while intestinal IL-6 levels showed a trend to significant increase 8 dpi. Strikingly, the lack of mMCP-4 resulted in significantly less intestinal transcriptional upregulation of IL-6, TNF-alpha, IL-25, CXCL2, IL-2, IL-4, IL-5, and IL-10 in the Giardia-infected mature adult mice, suggesting that chymase may play a regulatory role in intestinal cytokine responses.
Authors/Creators: | Li, Zhiqiang and Peirasmaki, Dimitra and Svaerd, Staffan and Åbrink, Magnus | ||||||
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Title: | The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalis | ||||||
Series Name/Journal: | Cells | ||||||
Year of publishing : | 2020 | ||||||
Volume: | 9 | ||||||
Article number: | 925 | ||||||
Number of Pages: | 19 | ||||||
Publisher: | MDPI | ||||||
ISSN: | 2073-4409 | ||||||
Language: | English | ||||||
Publication Type: | Research article | ||||||
Article category: | Scientific peer reviewed | ||||||
Version: | Published version | ||||||
Copyright: | Creative Commons: Attribution 4.0 | ||||||
Full Text Status: | Public | ||||||
Subjects: | (A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology | ||||||
Keywords: | mast cell, chymase, mMCP-4, infection, Giardia intestinalis | ||||||
URN:NBN: | urn:nbn:se:slu:epsilon-p-106502 | ||||||
Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106502 | ||||||
Additional ID: |
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ID Code: | 17192 | ||||||
Faculty: | VH - Faculty of Veterinary Medicine and Animal Science | ||||||
Department: | (VH) > Department of Biomedical Science and Veterinary Public Health | ||||||
Deposited By: | SLUpub Connector | ||||||
Deposited On: | 22 Jun 2020 13:40 | ||||||
Metadata Last Modified: | 15 Jan 2021 19:26 |
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