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Microstructural and carbohydrate compositional changes induced by enzymatic saccharification of green seaweed from West Africa

Thygesen, Anders and Ami, Johannes and Fernando, Dinesh and Bentil, Joseph and Daniel, Geoffrey and Mensah, Moses and Meyer, Anne S (2020). Microstructural and carbohydrate compositional changes induced by enzymatic saccharification of green seaweed from West Africa. Algal Research. 47 , 101894 , 1-9
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Abstract

The use of marine macroalgae as carbon source for fermentation is gaining increasing attention due to their high carbohydrate content and availability. Three green seaweed species Chaetomorpha linum, Ulva fasciata and Caulerpa taxifolia were investigated for their amenability to enzymatic saccharification. Microstructural changes were studied in order to understand the physical changes occurring in the seaweeds during saccarification and to identify structural barriers. C. linum had highest glucan content (20%), compared to 16% in U. fasciata and 6% in C. taxifolia indicating large differences in composition. Glucose yields obtained after 24 h of enzymatic saccharification were 59, 38 and 60% for C. taxifolia, U. fasciata and C. linum, respectively, based on the glucan content. Pre-autoclaving increased the saccharification yield to 81, 99 and 71%, respectively. Morphologically, C. linum displayed unbranched filaments, U. fasciata two-cell layer large sheets and C. taxifolia featured leaf like structures. Enzymatic saccharification resulted in cell wall degradation and release of the chlorophyll content in C. linum, delamination of sheets in U. fasciata and surface erosion of leaves in C. taxifolia. C. taxifolia deviated in being very rich in β-1,3 linked xylan (46%), which was only hydrolysed at 1% xylose yield due to lack of β-1,3-xylanase. Based on the high cellulose content and no need for pre-treatment C. linum was the best source of glucose among the three types of green seaweed species. Further studies are warranted to assess fermentation. We also conclude that for C. taxifolia enzymatic saccharification with β-1,3-xylanase might enable further xylose release than obtained here with the enzymatic cellulase treatment.

Authors/Creators:Thygesen, Anders and Ami, Johannes and Fernando, Dinesh and Bentil, Joseph and Daniel, Geoffrey and Mensah, Moses and Meyer, Anne S
Title:Microstructural and carbohydrate compositional changes induced by enzymatic saccharification of green seaweed from West Africa
Year of publishing :2020
Volume:47
Article number:101894
Number of Pages:9
Publisher:Elsevier
ISSN:2211-9264
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Accepted version
Copyright:Creative Commons: Attribution-Noncommercial-No Derivative Works 4.0
Full Text Status:Restricted
Subjects:(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 204 Chemical Engineering > Other Chemical Engineering
(A) Swedish standard research categories 2011 > 2 Engineering and Technology > 209 Industrial Biotechnology > Bio Materials
URN:NBN:urn:nbn:se:slu:epsilon-p-105917
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105917
Additional ID:
Type of IDID
DOI10.1016/j.algal.2020.101894
ID Code:17197
Faculty:S - Faculty of Forest Sciences
Department:(S) > Department of Forest Biomaterials and Technology
Deposited By: SLUpub Connector
Deposited On:03 Sep 2020 10:43
Metadata Last Modified:03 Sep 2020 10:43

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