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Fungi and Oomycetes in the Irrigation Water of Forest Nurseries

Marciulynas, Adas and Marciulyniene, Diana and Lynikiene, Jurate and Gedminas, Arturas and Vaiciukyne, Migle and Menkis, Audrius (2020). Fungi and Oomycetes in the Irrigation Water of Forest Nurseries. Forests. 11 , 459 , 1-16
[Journal article]

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Abstract

The aim of the present study was to assess fungal and oomycete communities in the irrigation water of forest nurseries, focusing on plant pathogens in the hope of getting a better understanding of potential pathogenic microorganisms and spreading routes in forest nurseries. The study sites were at Anykiai, Dubrava, Kretinga and Trakai state forest nurseries in Lithuania. For the collection of microbial samples, at each nursery five 100-L water samples were collected from the irrigation ponds and filtered. Following DNA isolation from the irrigation water filtrate samples, these were individually amplified using ITS rDNA as a marker and subjected to PacBio high-throughput sequencing. Clustering in the SCATA pipeline and the taxonomic classification of 24,006 high-quality reads showed the presence of 1286 non-singleton taxa. Among those, 895 were representing fungi and oomycetes. The detected fungi were 57.3% Ascomycota, 38.1% Basidiomycota, 3.1% Chytridiomycota, 0.8% Mucoromycota and 0.7% Oomycota. The most common fungi were Malassezia restricta E. Gueho, J. Guillot & Midgley (20.1% of all high-quality fungal sequences), Pezizella discreta (P. Karst.) Dennis (10.8%) and Epicoccum nigrum Link (4.9%). The most common oomycetes were Phytopythium cf. citrinum (B. Paul) Abad, de Cock, Bala, Robideau, Lodhi & Levesque (0.4%), Phytophthora gallica T. Jung & J. Nechwatal (0.05%) and Peronospora sp. 4248_322 (0.05%). The results demonstrated that the irrigation water used by forest nurseries was inhabited by a species-rich but largely site-specific communities of fungi. Plant pathogens were relatively rare, but, under suitable conditions, these can develop rapidly, spread efficiently through the irrigation system and be a threat to the production of high-quality tree seedlings.

Authors/Creators:Marciulynas, Adas and Marciulyniene, Diana and Lynikiene, Jurate and Gedminas, Arturas and Vaiciukyne, Migle and Menkis, Audrius
Title:Fungi and Oomycetes in the Irrigation Water of Forest Nurseries
Year of publishing :2020
Volume:11
Article number:459
Number of Pages:16
Publisher:MDPI
ISSN:1999-4907
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Forest Science
Keywords:fungal communities, oomycetes, irrigation water, seedlings pathogen, forest nurseries
URN:NBN:urn:nbn:se:slu:epsilon-p-106518
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106518
Additional ID:
Type of IDID
DOI10.3390/f11040459
Web of Science (WoS)000534632500097
ID Code:17200
Faculty:S - Faculty of Forest Sciences
Department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
(S) > Dept. of Forest Mycology and Plant Pathology
Deposited By: SLUpub Connector
Deposited On:29 Jun 2020 07:26
Metadata Last Modified:29 Jun 2020 07:26

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