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Lipid accumulation controls the balance between surface connection and scission of caveolae

Hubert, Madlen and Larsson, Elin and Vegesna, Naga Venkata Gayathri and Ahnlund, Maria and Johansson, Annika and Moodie, Lindon W. K. and Lundmark, Richard (2020). Lipid accumulation controls the balance between surface connection and scission of caveolae. eLife. 9 , e55038 , 1-31
[Journal article]

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Abstract

Caveolae are bulb-shaped invaginations of the plasma membrane (PM) that undergo scission and fusion at the cell surface and are enriched in specific lipids. However, the influence of lipid composition on caveolae surface stability is not well described or understood. Accordingly, we inserted specific lipids into the cell PM via membrane fusion and studied their acute effects on caveolae dynamics. We demonstrate that sphingomyelin stabilizes caveolae to the cell surface, whereas cholesterol and glycosphingolipids drive caveolae scission from the PM. Although all three lipids accumulated specifically in caveolae, cholesterol and sphingomyelin were actively sequestered, whereas glycosphingolipids diffused freely. The ATPase EHD2 restricts lipid diffusion and counteracts lipid-induced scission. We propose that specific lipid accumulation in caveolae generates an intrinsically unstable domain prone to scission if not restrained by EHD2 at the caveolae neck. This work provides a mechanistic link between caveolae and their ability to sense the PM lipid composition.

Authors/Creators:Hubert, Madlen and Larsson, Elin and Vegesna, Naga Venkata Gayathri and Ahnlund, Maria and Johansson, Annika and Moodie, Lindon W. K. and Lundmark, Richard
Title:Lipid accumulation controls the balance between surface connection and scission of caveolae
Year of publishing :2020
Volume:9
Article number:e55038
Number of Pages:31
Publisher:eLife Sciences Publications, Ltd
ISSN:2050-084X
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 106 Biological Sciences (Medical to be 3 and Agricultural to be 4) > Cell Biology
URN:NBN:urn:nbn:se:slu:epsilon-p-106653
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106653
Additional ID:
Type of IDID
DOI10.7554/eLife.55038
Web of Science (WoS)000537207600001
ID Code:17221
Faculty:S - Faculty of Forest Sciences
Department:(S) > Dept. of Forest Genetics and Plant Physiology
Deposited By: SLUpub Connector
Deposited On:10 Jul 2020 08:12
Metadata Last Modified:10 Jul 2020 08:12

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