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Efficient delipidation of a recombinant lung surfactant lipopeptide analogue by liquid-gel chromatography

Basabe-Burgos, Oihana and Zebialowicz Ahlström, Jakub and Mikolka, Pavol and Landreh, Michael and Johansson, Jan and Curstedt, Tore and Rising, Anna (2019). Efficient delipidation of a recombinant lung surfactant lipopeptide analogue by liquid-gel chromatography. PLoS ONE. 14 , e0226072 , 1-12
[Journal article]

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Abstract

Pulmonary surfactant preparations extracted from natural sources have been used to treat millions of newborn babies with respiratory distress syndrome (RDS) and can possibly also be used to treat other lung diseases. Due to costly production and limited supply of animal-derived surfactants, synthetic alternatives are attractive. The water insolubility and aggregation-prone nature of the proteins present in animal-derived surfactant preparations have complicated development of artificial surfactant. A non-aggregating analog of lung surfactant protein C, SP-C33Leu is used in synthetic surfactant and we recently described an efficient method to produce rSP-C33Leu in bacteria. Here rSP-C33Leu obtained by salt precipitation of bacterial extracts was purified by two-step liquid gel chromatography and analyzed using mass spectrometry and RP-HPLC, showing that it is void of modifications and adducts. Premature New Zealand White rabbit fetuses instilled with 200mg/kg of 2% of rSP-C33Leu in phospholipids and ventilated with a positive end expiratory pressure showed increased tidal volumes and lung gas volumes compared to animals treated with phospholipids only. This shows that rSP-C33Leu can be purified from bacterial lipids and that rSP-C33Leu surfactant is active against experimental RDS.

Authors/Creators:Basabe-Burgos, Oihana and Zebialowicz Ahlström, Jakub and Mikolka, Pavol and Landreh, Michael and Johansson, Jan and Curstedt, Tore and Rising, Anna
Title:Efficient delipidation of a recombinant lung surfactant lipopeptide analogue by liquid-gel chromatography
Year of publishing :2019
Volume:14
Article number:e0226072
Number of Pages:12
Publisher:PLoS
ISSN:1932-6203
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 1 Natural sciences > 104 Chemical Sciences > Analytical Chemistry
URN:NBN:urn:nbn:se:slu:epsilon-p-105882
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-105882
Additional ID:
Type of IDID
DOI10.1371/journal.pone.0226072
Web of Science (WoS)000533987600062
ID Code:17227
Faculty:VH - Faculty of Veterinary Medicine and Animal Science
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Deposited By: SLUpub Connector
Deposited On:10 Jul 2020 09:46
Metadata Last Modified:10 Jul 2020 09:46

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