Home About Browse Search
Svenska


Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani.

Ali, Ashfaq and Alexandersson, Erik and Sandin, Marianne and Lenman, Marit and Resjö, Svante and Hedley, Pete and Levander, Fredrik and Andreasson, Erik (2014). Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani. BMC Genomics. 15 , 497 , 1-18
[Journal article]

[img] PDF
2MB

Abstract

Background
In order to get global molecular understanding of one of the most important crop diseases worldwide, we investigated compatible and incompatible interactions between Phytophthora infestans and potato (Solanum tuberosum). We used the two most field-resistant potato clones under Swedish growing conditions, which have the greatest known local diversity of P. infestans populations, and a reference compatible cultivar.
Results
Quantitative label-free proteomics of 51 apoplastic secretome samples (PXD000435) in combination with genome-wide transcript analysis by 42 microarrays (E-MTAB-1515) were used to capture changes in protein abundance and gene expression at 6, 24 and 72 hours after inoculation with P. infestans. To aid mass spectrometry analysis we generated cultivar-specific RNA-seq data (E-MTAB-1712), which increased peptide identifications by 17%. Components induced only during incompatible interactions, which are candidates for hypersensitive response initiation, include a Kunitz-like protease inhibitor, transcription factors and an RCR3-like protein. More secreted proteins had lower abundance in the compatible interaction compared to the incompatible interactions. Based on this observation and because the well-characterized effector-target C14 protease follows this pattern, we suggest 40 putative effector targets.
Conclusions
In summary, over 17000 transcripts and 1000 secreted proteins changed in abundance in at least one time point, illustrating the dynamics of plant responses to a hemibiotroph. Half of the differentially abundant proteins showed a corresponding change at the transcript level. Many putative hypersensitive and effector-target proteins were single representatives of large gene families.

Authors/Creators:Ali, Ashfaq and Alexandersson, Erik and Sandin, Marianne and Lenman, Marit and Resjö, Svante and Hedley, Pete and Levander, Fredrik and Andreasson, Erik
Title:Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani.
Year of publishing :2014
Volume:15
Article number:497
Number of Pages:18
Publisher:BMC
ISSN:1471-2164
Language:English
Publication Type:Journal article
Article category:Scientific peer reviewed
Version:Published version
Copyright:Creative Commons: Attribution 4.0
Full Text Status:Public
Subjects:(A) Swedish standard research categories 2011 > 4 Agricultural Sciences > 401 Agricultural, Forestry and Fisheries > Agricultural Science
Keywords:Potato, Desiree, Sarpo Mira, SW93-1015, Secretome, Apoplast, Resistance, Hypersensitive response, Phytophthora infestans
URN:NBN:urn:nbn:se:slu:epsilon-p-106699
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-p-106699
Additional ID:
Type of IDID
DOI10.1186/1471-2164-15-497
ID Code:17261
Faculty:LTV - Fakulteten för landskapsarkitektur, trädgårds- och växtproduktionsvetenskap
Department:(LTJ, LTV) > Department of Plant Protection Biology
Deposited By: SLUpub Connector
Deposited On:18 Aug 2020 07:04
Metadata Last Modified:18 Aug 2020 07:04

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits